Human Receptor-type tyrosine-protein kinase FLT3 ELISA Kit | FLT3 elisa kit

Human Receptor-type tyrosine-protein kinase FLT3 ELISA Kit

Cat. Num. AAA765646

• Gene Names: FLT3; FLK2; STK1; CD135; FLK-2
• Reactivity: Human
• Synonyms: Receptor-type tyrosine-protein kinase FLT3; Human Receptor-type tyrosine-protein kinase FLT3 ELISA Kit; Flt3 (FMS Like Tyrosine Kinase 3)/CD135/Flk-2/STK-1/CD135/CD135 antigen/fetal liver kinase 2/FL cytokine receptor/FLK2/FLT-3/FLT3 receptor tyrosine kinase/Fms-like tyrosine kinase 3/fms-related tyrosine kinase 3/growth factor receptor tyrosine kinase type III/Stem cell tyrosine kinase 1; FLT3 elisa kit

• Reactivity: Human
• Sequence Length: 993

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for FLT3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human Flt3antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human Flt3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Flt3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman Flt3can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 121114304
• NCBI GeneID: 2322
• NCBI Accession #: NP_004110.2
• NCBI GenBank Nucleotide #: NM_004119.2
• Molecular Weight: 108,378 Da
• NCBI Official Full Name: receptor-type tyrosine-protein kinase FLT3
• NCBI Official Synonym Full Names: fms related tyrosine kinase 3
• NCBI Official Symbol: FLT3
• NCBI Official Synonym Symbols: FLK2; STK1; CD135; FLK-2
• NCBI Protein Information: receptor-type tyrosine-protein kinase FLT3
• UniProt Protein Name: Receptor-type tyrosine-protein kinase FLT3
• Protein Family: Receptor-type tyrosine-protein kinase
• UniProt Gene Name: FLT3
• UniProt Synonym Gene Names: CD135; FLK2; STK1; FLK-2; FLT-3; STK-1
• UniProt Entry Name: FLT3_HUMAN

NCBI Description

This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia. [provided by RefSeq, Jan 2015]

Uniprot Description

FLT3: a receptor tyrosine kinase of the PDGFR family that binds the FL cytokine. FLT3 -/- mice have an impaired developmental capacity of primitive hematopoietic progenitor cells of all lineages with the greatest impact on lymphopoietic precursors. Activating mutations found in one third of cases of acute myeloid leukemia (AML), as well as in acute lymphoblastic leukemia, acute promyelocytic leukemia and myelodysplastic syndrome. Inhibitors: Sutent and PKC412.

Protein type: Protein kinase, tyrosine (receptor); EC 2.7.10.1; Protein kinase, TK; Oncoprotein; Kinase, protein; Membrane protein, integral; TK group; PDGFR family

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: cytosol; endoplasmic reticulum lumen; external side of plasma membrane; integral to plasma membrane; nucleus; protein complex

Molecular Function: ATP binding; glucocorticoid receptor binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; phosphoinositide 3-kinase binding; protein binding; protein complex binding; protein homodimerization activity; transmembrane receptor protein tyrosine kinase activity; vascular endothelial growth factor receptor activity

Biological Process: B cell differentiation; cytokine and chemokine mediated signaling pathway; hemopoiesis; homeostasis of number of cells within a tissue; leukocyte homeostasis; lymphocyte proliferation; myeloid progenitor cell differentiation; negative regulation of B cell differentiation; negative regulation of cell proliferation; organ regeneration; peptidyl-tyrosine phosphorylation; positive regulation of cell proliferation; positive regulation of MAP kinase activity; positive regulation of MAPKKK cascade; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of tyrosine phosphorylation of STAT protein; post-embryonic development; pro-B cell differentiation; pro-T cell differentiation; protein amino acid autophosphorylation; regulation of apoptosis; response to organic nitrogen; spleen development; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Leukemia, Acute Lymphoblastic