Rat Fibroblast Growth Factor 4 ELISA Kit | FGF4 elisa kit

Rat Fibroblast Growth Factor 4 ELISA Kit

Cat. Num. AAA763616

• Gene Names: Fgf4; KS3; hst; Fgfk; Hst1; kFGF; Fgf-4; hst-1; Hstf-1
• Reactivity: Rat
• Synonyms: Fibroblast Growth Factor 4; Rat Fibroblast Growth Factor 4 ELISA Kit; FGF4/FGF-4; FGF4 elisa kit

• Reactivity: Rat
• Sequence Length: 202

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for FGF4 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat FGF4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat FGF4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat FGF4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat FGF4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 66955870
• NCBI GeneID: 14175
• NCBI Accession #: NP_034332.2
• NCBI GenBank Nucleotide #: NM_010202.5
• Molecular Weight: 21,919 Da
• NCBI Official Full Name: fibroblast growth factor 4
• NCBI Official Synonym Full Names: fibroblast growth factor 4
• NCBI Official Symbol: Fgf4
• NCBI Official Synonym Symbols: KS3; hst; Fgfk; Hst1; kFGF; Fgf-4; hst-1; Hstf-1
• NCBI Protein Information: fibroblast growth factor 4
• UniProt Protein Name: Fibroblast growth factor 4
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: Fgf4
• UniProt Synonym Gene Names: Fgf-4; Kfgf; FGF-4; HBGF-4
• UniProt Entry Name: FGF4_MOUSE

Uniprot Description

FGF4: Plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. Required for normal limb and cardiac valve development during embryogenesis. Belongs to the heparin-binding growth factors family.

Protein type: Secreted, signal peptide; Secreted; Oncoprotein; Cytokine

Cellular Component: extracellular region

Molecular Function: fibroblast growth factor receptor binding; growth factor activity; protein binding; receptor binding

Biological Process: cartilage condensation; cell differentiation; embryonic hindlimb morphogenesis; embryonic limb morphogenesis; fibroblast growth factor receptor signaling pathway; mesenchymal cell proliferation; multicellular organismal development; negative regulation of apoptosis; odontogenesis of dentine-containing teeth; positive regulation of cell division; positive regulation of cell proliferation; positive regulation of protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase II promoter; regulation of gene expression; stem cell maintenance