Mouse Defensin Alpha 5, Paneth Cell Specific (DEFa5) ELISA Kit | DEFa5 elisa kit

Mouse Defensin Alpha 5, Paneth Cell Specific (DEFa5) ELISA Kit

Cat. Num. AAA2703886

• Gene Names: DEFA5; DEF5; HD-5
• Reactivity: Mouse
• Synonyms: Defensin Alpha 5; Paneth Cell Specific (DEFa5); Mouse Defensin Alpha 5; Paneth Cell Specific (DEFa5) ELISA Kit; DEF-A5; DEF5; HD-5; DEFa5 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of DEFa5. No significant cross-reactivity or interference between DEFa5 and analogues was observed.
• Sequence Length: 94

• Samples: Human Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: 6.3pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DEFa5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DEFa5 were tested on 3 different plates, 8 replicates in each plate.CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DEFa5 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DEFa5 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to DEFa5. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to DEFa5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DEFa5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DEFa5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 50057850
• NCBI GeneID: 1670
• Molecular Weight: 10,071 Da
• NCBI Official Full Name: defensin, alpha 5, Paneth cell-specific
• NCBI Official Synonym Full Names: defensin alpha 5
• NCBI Official Symbol: DEFA5
• NCBI Official Synonym Symbols: DEF5; HD-5
• NCBI Protein Information: defensin-5
• UniProt Protein Name: Defensin-5
• Protein Family: Defensin
• UniProt Gene Name: DEFA5
• UniProt Synonym Gene Names: DEF5

NCBI Description

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several of the alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 5, is highly expressed in the secretory granules of Paneth cells of the ileum. [provided by RefSeq, Oct 2014]

Uniprot Description

DEFA5: Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing. Belongs to the alpha-defensin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular region; extracellular space; Golgi lumen; transport vesicle

Molecular Function: protein homodimerization activity

Biological Process: antimicrobial humoral response; defense response to fungus; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; innate immune response; killing of cells of other organism