Human Defensin Alpha 5, Paneth Cell Specific ELISA Kit | DEFA5 elisa kit

Human Defensin Alpha 5, Paneth Cell Specific ELISA Kit

Cat. Num. AAA761731

• Gene Names: DEFA5; DEF5; HD-5
• Reactivity: Human
• Synonyms: Defensin Alpha 5; Paneth Cell Specific; Human Defensin Alpha 5; Paneth Cell Specific ELISA Kit; DEFA5/Defensin-5/Defensin; alpha 5/HD5 (20-94)/DEF5/HD-5; DEFA5 elisa kit

• Reactivity: Human
• Sequence Length: 94

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for DEFA5 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-DEFA5 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-DEFA5 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the DEFA5 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of DEFA5 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 50057850
• NCBI GeneID: 1670
• Molecular Weight: 10,071 Da
• NCBI Official Full Name: defensin, alpha 5, Paneth cell-specific
• NCBI Official Synonym Full Names: defensin alpha 5
• NCBI Official Symbol: DEFA5
• NCBI Official Synonym Symbols: DEF5; HD-5
• NCBI Protein Information: defensin-5
• UniProt Protein Name: Defensin-5
• Protein Family: Defensin
• UniProt Gene Name: DEFA5
• UniProt Synonym Gene Names: DEF5
• UniProt Entry Name: DEF5_HUMAN

NCBI Description

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several of the alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 5, is highly expressed in the secretory granules of Paneth cells of the ileum. [provided by RefSeq, Oct 2014]

Uniprot Description

DEFA5: Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing. Belongs to the alpha-defensin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular region; extracellular space; Golgi lumen; transport vesicle

Biological Process: antibacterial humoral response; defense response to fungus; innate immune response; killing of cells of another organism