Mouse Death Associated Protein Kinase 1 (DAPK1) ELISA Kit | DAPK1 elisa kit

Mouse Death Associated Protein Kinase 1 (DAPK1) ELISA Kit

Cat. Num. AAA2019499

• Gene Names: DAPK1; DAPK
• Reactivity: Mouse
• Synonyms: Death Associated Protein Kinase 1 (DAPK1); Mouse Death Associated Protein Kinase 1 (DAPK1) ELISA Kit; DAPK1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Death Associated Protein Kinase 1 (DAPK1).; No significant cross-reactivity or interference between Death Associated Protein Kinase 1 (DAPK1) and analogues was observed.
• Sequence Length: 1430

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.051ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Death Associated Protein Kinase 1 (DAPK1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Death Associated Protein Kinase 1 (DAPK1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DAPK1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Death Associated Protein Kinase 1 (DAPK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Death Associated Protein Kinase 1 (DAPK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Death Associated Protein Kinase 1 (DAPK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Death Associated Protein Kinase 1 (DAPK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for DAPK1 elisa kit

Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 89363047
• NCBI GeneID: 1612
• NCBI Accession #: NP_004929.2
• NCBI GenBank Nucleotide #: NM_004938.3
• UniProt Accession #: P53355; Q14CQ7; Q1W5W0; Q68CP8; Q6ZRZ3; Q9BTL8; B7ZLD2; B7ZLE7
• Molecular Weight: 152,467 Da
• NCBI Official Full Name: death-associated protein kinase 1
• NCBI Official Synonym Full Names: death-associated protein kinase 1
• NCBI Official Symbol: DAPK1
• NCBI Official Synonym Symbols: DAPK
• NCBI Protein Information: death-associated protein kinase 1
• UniProt Protein Name: Death-associated protein kinase 1
• Protein Family: Death-associated protein kinase
• UniProt Gene Name: DAPK1
• UniProt Synonym Gene Names: DAPK; DAP kinase 1
• UniProt Entry Name: DAPK1_HUMAN

NCBI Description

Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

DAPK1: a CAMK protein kinase of the Death-associated protein kinase (DAPK) family. Mediates autophagy and is a positive mediator of ER stress-induced and gamma-interferon induced programmed cell death. Catalytic activity is increased by ER stress. Inhibited by autophosphorylation of S308. DAPK -/- cells are protected from ER stress-induced cell death, and both apoptosis and autophagy are decreased. Colocalizes with the actin filament system. It is a tumor suppressor candidate, activating p53-dependent apoptosis. Expression is reduced in some cancers, and the promoter is frequently hypermethylated in invasive cancers. Increased expression and mislocalization is seen in epilepsy, where it may regulate neuronal death. A structurally unique serine-threonine kinase that carries eight ankyrin repeats and two putative P-loop consensus sites. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, CAMK; EC 2.7.11.1; Autophagy; Apoptosis; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CAMK group; DAPK family

Chromosomal Location of Human Ortholog: 9q21.33

Cellular Component: cytoplasm; plasma membrane; actin cytoskeleton

Molecular Function: calmodulin binding; protein serine/threonine kinase activity; identical protein binding; protein binding; calmodulin-dependent protein kinase activity; syntaxin-1 binding; ATP binding; protein kinase activity

Biological Process: regulation of apoptosis; induction of apoptosis via death domain receptors; positive regulation of apoptosis; apoptosis; protein amino acid autophosphorylation; negative regulation of translation; positive regulation of caspase activity; regulation of autophagy; protein amino acid phosphorylation