Rabbit anti-Dog, Human DAPK1 / DAP Kinase Polyclonal Antibody | anti-DAPK1 antibody

Rabbit Polyclonal (IgG) to Human DAPK1 / DAP Kinase

Cat. Num. AAA248708

• Gene Names: DAPK1; DAPK
• Reactivity: Dog, Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: DAPK1 / DAP Kinase; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human DAPK1 / DAP Kinase; Anti-DAPK1 / DAP Kinase Antibody (aa38-374) IHC-plus; DAPK1; DAP kinase 1; DAPK; Human DAPK1; DAP Kinase; anti-DAPK1 antibody

• Host: Rabbit
• Reactivity: Dog, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human DAPK1 / DAP Kinase.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.1 M Tris-glycine, pH 7, 20% Glycerol, 0.01% Thimerosal
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1430

• Applicable Applications for anti-DAPK1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (5 - 10 ug/ml), WB
• Target Species: Human
• Immunogen Description: Recombinant fragment corresponding to a region within amino acids 38 and 374 of DAP Kinase 1 (SwissProt P53355). Percent identity by BLAST analysis: Human, Dog, Rhesus monkey (100%).
• Immunogen Type: Recombinant protein
• Immunogen: DAPK1 / DAP Kinase antibody was raised against recombinant fragment corresponding to a region within amino acids 38 and 374 of DAP Kinase 1 (SwissProt P53355). Percent identity by BLAST analysis: Human, Dog, Rhesus monkey (100%).
• Antigen Modification: aa38-374
• Preparation and Storage: Keep as concentrated solution. Aliquot and store at -20 degree C or below. Avoid multiple freeze-thaw cycles.

Immunohistochemistry (IHC)

(Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))

Related Product Information for anti-DAPK1 antibody

DAPK1, a DAPK type protein kinase, is a positive mediator of gamma-interferon induced programmed cell death. It is believed to function in an early apoptotic checkpoint designed to eliminate premalignant cells from cancer development. The DAPK1 protein contains eight ankyrin repeats and two putative P-loop consensus sites. Loss of DAPK1 expression has been associated with invasive tumors.

Product Categories/Family for anti-DAPK1 antibody

Family: Protein Kinase; Subfamily: DAPK

NCBI and Uniprot Product Information

• NCBI GI #: 89363047
• NCBI GeneID: 1612
• NCBI Accession #: NP_004929.2
• NCBI GenBank Nucleotide #: NM_004938.3
• UniProt Accession #: P53355; Q14CQ7; Q1W5W0; Q68CP8; Q6ZRZ3; Q9BTL8; B7ZLD2; B7ZLE7
• Molecular Weight: 152,467 Da
• NCBI Official Full Name: death-associated protein kinase 1
• NCBI Official Synonym Full Names: death-associated protein kinase 1
• NCBI Official Symbol: DAPK1
• NCBI Official Synonym Symbols: DAPK
• NCBI Protein Information: death-associated protein kinase 1; DAP kinase 1
• UniProt Protein Name: Death-associated protein kinase 1
• Protein Family: Death-associated protein kinase
• UniProt Gene Name: DAPK1
• UniProt Synonym Gene Names: DAPK; DAP kinase 1
• UniProt Entry Name: DAPK1_HUMAN

NCBI Description

Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

DAPK1: a CAMK protein kinase of the Death-associated protein kinase (DAPK) family. Mediates autophagy and is a positive mediator of ER stress-induced and gamma-interferon induced programmed cell death. Catalytic activity is increased by ER stress. Inhibited by autophosphorylation of S308. DAPK -/- cells are protected from ER stress-induced cell death, and both apoptosis and autophagy are decreased. Colocalizes with the actin filament system. It is a tumor suppressor candidate, activating p53-dependent apoptosis. Expression is reduced in some cancers, and the promoter is frequently hypermethylated in invasive cancers. Increased expression and mislocalization is seen in epilepsy, where it may regulate neuronal death. A structurally unique serine-threonine kinase that carries eight ankyrin repeats and two putative P-loop consensus sites. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; EC 2.7.11.1; Apoptosis; Protein kinase, CAMK; Autophagy; Protein kinase, Ser/Thr (non-receptor); CAMK group; DAPK family

Chromosomal Location of Human Ortholog: 9q21.33

Cellular Component: cytoplasm; plasma membrane; actin cytoskeleton

Molecular Function: calmodulin binding; protein serine/threonine kinase activity; identical protein binding; protein binding; calmodulin-dependent protein kinase activity; syntaxin-1 binding; ATP binding; protein kinase activity

Biological Process: regulation of apoptosis; induction of apoptosis via death domain receptors; apoptosis; positive regulation of apoptosis; negative regulation of translation; protein amino acid autophosphorylation; positive regulation of caspase activity; regulation of autophagy; protein amino acid phosphorylation