Human death-associated protein kinase 1 ELISA Kit | DAPK1 elisa kit

Human death-associated protein kinase, DAPK ELISA Kit

Cat. Num. AAA702602

• Gene Names: DAPK1; DAPK
• Reactivity: Human
• Synonyms: death-associated protein kinase 1; Human death-associated protein kinase; DAPK ELISA Kit; Human death-associated protein kinase (DAPK) ELISA Kit; DAPK; DKFZp781I035; ; DAPK1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human DAF/CD55. No significant cross-reactivity or interference between human DAF/CD55 and analogues was observed.
• Sequence Length: 1430

• Samples: Serum, Plasma, Cell Culture Supernates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.625 ng/ml-40 ng/ml
• Sensitivity: 0.156 ng/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DAPK1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DAF/CD55 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DAF/CD55 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DAF/CD55 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DAF/CD55 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 570359575
• NCBI GeneID: 1612
• NCBI Accession #: NP_001275658.1
• NCBI GenBank Nucleotide #: NM_001288729.1
• UniProt Accession #: P53355; Q14CQ7; Q1W5W0; Q68CP8; Q6ZRZ3; Q9BTL8; B7ZLD2; B7ZLE7
• Molecular Weight: 160,046 Da
• NCBI Official Full Name: death-associated protein kinase 1
• NCBI Official Synonym Full Names: death-associated protein kinase 1
• NCBI Official Symbol: DAPK1
• NCBI Official Synonym Symbols: DAPK
• NCBI Protein Information: death-associated protein kinase 1; DAP kinase 1
• UniProt Protein Name: Death-associated protein kinase 1
• Protein Family: Death-associated protein kinase
• UniProt Gene Name: DAPK1
• UniProt Synonym Gene Names: DAPK; DAP kinase 1
• UniProt Entry Name: DAPK1_HUMAN

NCBI Description

Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

Function: Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca2+ influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.15 PublicationsManual assertion based on experiment in:Ref.1

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium.

Enzyme regulation: Activated by Ca2+/calmodulin. Regulated by a locking mechanism, involving autophosphorylation at Ser-308 and calmodulin binding. In the inactive state, Ser-308 is phosphorylated. Activation involves its dephosphorylation and a release-of-autoinhibition mechanism where binding of calmodulin induces a conformational change that relieves the steric block of the active site by the autoinhibitory domain. Activity is modulated by UNC5B and NTN1. UNC5B activates it by inhibiting the phosphorylation at Ser-308, whereas NTN1 inhibits UNC5B-mediated activation of DAPK1. Endoplasmic-stress activates by causing Ser-308 dephosphorylation.3 PublicationsManual assertion based on experiment in:Ref.11