Immunohistochemistry (IHC)
(Anti-KDM1B antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Rabbit anti-Mouse, Human KDM1B Polyclonal Antibody | anti-KDM1B antibody
Anti-KDM1B Antibody (N-Terminus) IHC-plus
WB (1 - 2 ug/ml)
Short term: 4 degree C
Avoid repeat freeze-thaw cycles
Immunohistochemistry (IHC)
(Anti-KDM1B antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunohistochemistry (IHC)
(Anti-KDM1B antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Western Blot (WB)
(Western blot analysis of KDM1B in 3T3 cell lysate with KDM1B antibody at 2 ug/ml in (A) the absence and (B) the presence of blocking peptide.)
Western Blot (WB)
(Western blot analysis of KDM1B in 3T3 cell lysate with KDM1B antibody at 2 ug/ml in (A) the absence and (B) the presence of blocking peptide.)
NCBI and Uniprot Product Information
NCBI Description
Flavin-dependent histone demethylases, such as KDM1B, regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function (Karytinos et al., 2009 [PubMed 19407342]).[supplied by OMIM, Oct 2009]
Uniprot Description
AOF1: Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri- methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys- 36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4. Belongs to the flavin monoamine oxidase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Oxidoreductase; EC 1.-.-.-
Chromosomal Location of Human Ortholog: 6p22.3
Cellular Component: nucleoplasm; nucleus
Molecular Function: DNA binding; FAD binding; zinc ion binding
Biological Process: DNA methylation during gametogenesis; establishment and/or maintenance of chromatin architecture; genetic imprinting; multicellular organismal development; regulation of transcription, DNA-dependent; transcription, DNA-dependent
Research Articles on KDM1B
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Product Notes
The KDM1B kdm1b (Catalog #AAA249936) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-KDM1B Antibody (N-Terminus) IHC-plus reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's KDM1B can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA). IHC-P (10 ug/ml) WB (1 - 2 ug/ml). Researchers should empirically determine the suitability of the KDM1B kdm1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KDM1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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