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Typical Testing Data/Standard Curve (for reference only)

Mouse Corticotropin Releasing Factor (CRF) ELISA Kit | CRF elisa kit

Mini Samples Corticotropin Releasing Factor (CRF) ELISA Kit

Gene Names
Crh; CRF; Gm1347
Reactivity
Mouse
Applications
ELISA
Synonyms
Corticotropin Releasing Factor (CRF); Mini Samples Corticotropin Releasing Factor (CRF) ELISA Kit; CRH; Corticotropin Releasing Hormone; Corticoliberin; CRF elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of CRF. No significant cross-reactivity or interference between CRF and analogues was observed.
Applicable Applications for CRF elisa kit
ELISA (EIA)
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Competitive
Detection Range
12.35-1,000pg/mL
Sensitivity
< 5.37pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CRF were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CRF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CRF elisa kit
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of CRF in mouse serum, plasma and other biological fluids.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to CRF has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled CRF and unlabeled CRF (Standards or samples) with the pre-coated antibody specific to CRF. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of CRF in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of CRF in the sample.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20,778 Da
NCBI Official Full Name
corticoliberin preproprotein
NCBI Official Synonym Full Names
corticotropin releasing hormone
NCBI Official Symbol
Crh
NCBI Official Synonym Symbols
CRF; Gm1347
NCBI Protein Information
corticoliberin
UniProt Protein Name
Corticoliberin
UniProt Gene Name
Crh
UniProt Synonym Gene Names
Gm1347; CRF

NCBI Description

This gene encodes a member of the corticotropin-releasing factor family and preproprotein that is proteolytically processed to generate a mature protein product. This protein product is a neuropeptide hormone that binds to the corticotropin releasing hormone receptors (CRHR1 and CRHR2) to stimulate the release of adrenocorticotropic hormone from the pituitary gland in response to stress. The encoded protein may also regulate angiogenesis and inflammation. Homozygous knockout mice for this gene exhibit reduced corticosterone levels while the offspring of these mice die perinatally. [provided by RefSeq, Aug 2015]

Uniprot Description

Hormone regulating the release of corticotropin from pituitary gland. Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile (PubMed:23470617).

Research Articles on CRF

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Product Notes

The Mouse CRF crh (Catalog #AAA2709406) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2709406 ELISA Kit recognizes Mouse CRF. AAA Biotech's Corticotropin Releasing Factor (CRF) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the CRF crh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Corticotropin Releasing Factor (CRF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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