Typical Testing Data/Standard Curve (for reference only)
Human Corticotropin Releasing Hormone (CRH) ELISA Kit | CRH elisa kit
Human Corticotropin Releasing Hormone (CRH) ELISA Kit
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to cotinine has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled cotinine and unlabeled cotinine (Standards or samples) with the pre-coated antibody specific to cotinine. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of cotinine in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of cotinine in the sample.
NCBI and Uniprot Product Information
NCBI Description
Corticotropin-releasing hormone is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in this protein has been observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin deficiency has multiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturition. [provided by RefSeq, Apr 2010]
Uniprot Description
CRH: This hormone from hypothalamus regulates the release of corticotropin from pituitary gland. Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 8q13
Cellular Component: extracellular space; cytoplasm; extracellular region; perikaryon
Molecular Function: corticotropin-releasing hormone receptor 1 binding; neuropeptide hormone activity; protein binding; hormone activity; corticotropin-releasing hormone receptor 2 binding; receptor binding; adrenocorticotropin-releasing hormone activity
Biological Process: synaptic transmission, dopaminergic; diterpenoid metabolic process; negative regulation of circadian sleep/wake cycle, REM sleep; hypothalamus development; positive regulation of cortisol secretion; induction of apoptosis by hormones; response to pain; female pregnancy; signal transduction; positive regulation of adrenocorticotropic hormone secretion; synaptic transmission; negative regulation of luteinizing hormone secretion; learning and/or memory; positive regulation of cell proliferation; positive regulation of cAMP biosynthetic process; feeding behavior; negative regulation of blood pressure; parturition; response to electrical stimulus; inflammatory response; associative learning; response to corticosterone stimulus; response to drug; adrenal gland development; response to ether; response to ethanol; long-term memory; ion homeostasis; response to estrogen stimulus; regulation of serotonin secretion; negative regulation of epinephrine secretion; positive regulation of protein amino acid phosphorylation; glucocorticoid biosynthetic process; lung development
Disease: Acth Deficiency, Isolated
Research Articles on CRH
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Product Notes
The Human CRH crh (Catalog #AAA2023613) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2023613 ELISA Kit recognizes Human CRH. It is sometimes possible for the material contained within the vial of "Corticotropin Releasing Hormone (CRH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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