Mouse CLCF1 ELISA Kit | CLCF1 elisa kit
Mouse CLCF1 (Cardiotrophin Like Cytokine Factor 1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CLCF1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse CLCF1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CLCF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CLCF1. You can calculate the concentration of Mouse CLCF1 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the glycoprotein (gp)130 cytokine family and encodes cardiotrophin-like cytokine factor 1 (CLCF1). CLCF1 forms a heterodimer complex with cytokine receptor-like factor 1 (CRLF1). This dimer competes with ciliary neurotrophic factor (CNTF) for binding to the ciliary neurotrophic factor receptor (CNTFR) complex, and activates the Jak-STAT signaling cascade. CLCF1 can be actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR. CLCF1 is a potent neurotrophic factor, B-cell stimulatory agent and neuroendocrine modulator of pituitary corticotroph function. Defects in CLCF1 cause cold-induced sweating syndrome 2 (CISS2). This syndrome is characterized by a profuse sweating after exposure to cold as well as congenital physical abnormalities of the head and spine. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009]
Uniprot Description
CLCF1: Cytokine with B-cell stimulating capability. Binds to and activates the ILST/gp130 receptor. Defects in CLCF1 are the cause of cold-induced sweating syndrome type 2 (CISS2). Cold-induced sweating syndrome (CISS) is an autosomal recessive disorder characterized by profuse sweating induced by cool surroundings (temperatures of 7 to 18 degrees Celsius). Additional abnormalities include a high- arched palate, nasal voice, depressed nasal bridge, inability to fully extend the elbows and kyphoscoliosis. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Apoptosis; Cytokine; Secreted
Chromosomal Location of Human Ortholog: 11q13.3
Cellular Component: extracellular region
Molecular Function: protein binding; growth factor activity; protein heterodimerization activity; cytokine activity; ciliary neurotrophic factor receptor binding; receptor binding
Biological Process: positive regulation of immunoglobulin production; cell surface receptor linked signal transduction; cytokine and chemokine mediated signaling pathway; B cell differentiation; positive regulation of cell proliferation; positive regulation of B cell proliferation; negative regulation of neuron apoptosis; JAK-STAT cascade; positive regulation of isotype switching to IgE isotypes; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of astrocyte differentiation
Disease: Cold-induced Sweating Syndrome 2
