Rabbit PKC zeta Polyclonal Antibody | anti-PKC zeta antibody

Rabbit polyclonal PKC zeta antibody

Cat. Num. AAA5304964

• Gene Names: PRKCZ; PKC2; PKC-ZETA
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by immunoaffinity chromatography.
• Synonyms: PKC zeta; Polyclonal Antibody; Rabbit polyclonal PKC zeta antibody; PKC zeta antibody; anti-PKC zeta antibody

• Host: Rabbit
• Clonality: Polyclonal
• Purity/Purification: Purified by immunoaffinity chromatography.
• Form/Format: Supplied in liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 592

• Applicable Applications for anti-PKC zeta antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; WB: 1:500-1:1000
• Immunogen: PKC zeta antibody was raised in Rabbit using the peptide sequence around aa 408~412 (T-S-T-F-C) derived from PKCzeta as the immunogen.
• Cross-Reactivity: Human, Mouse, Rat
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKC antibody)

Product Categories/Family for anti-PKC zeta antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 52486327
• NCBI GeneID: 5590
• NCBI Accession #: NP_002735.3
• NCBI GenBank Nucleotide #: NM_002744.4
• UniProt Accession #: Q05513; Q15207; Q5SYT5; Q969S4; A8K4N0; A8MU64; B7Z2J7; E9PCW2
• Molecular Weight: 56,136 Da
• NCBI Official Full Name: protein kinase C zeta type isoform 1
• NCBI Official Synonym Full Names: protein kinase C zeta
• NCBI Official Symbol: PRKCZ
• NCBI Official Synonym Symbols: PKC2; PKC-ZETA
• NCBI Protein Information: protein kinase C zeta type
• UniProt Protein Name: Protein kinase C zeta type
• UniProt Gene Name: PRKCZ
• UniProt Synonym Gene Names: PKC2

NCBI Description

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCZ: an AGC kinase of the PKC family. An atypical PKC: its activity is not regulated by Ca2+, PS, DAG or phorbol esters. Constitutively active.

Protein type: AGC group; EC 2.7.11.13; Iota subfamily; Kinase, protein; PKC family; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: cell junction; cytoplasm; cytosol; intercellular junction; membrane; plasma membrane; vesicle

Molecular Function: insulin receptor substrate binding; protein binding; protein kinase activity; protein kinase C activity; protein serine/threonine kinase activity

Biological Process: activation of NF-kappaB transcription factor; establishment of cell polarity; negative regulation of insulin receptor signaling pathway; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of protein complex assembly; peptidyl-serine phosphorylation; positive regulation of insulin receptor signaling pathway; positive regulation of interleukin-4 production; positive regulation of T-helper 2 cell differentiation; protein amino acid phosphorylation; signal transduction; transforming growth factor beta receptor signaling pathway