Human C-Reactive Protein ELISA Kit | hsCRP elisa kit

C-Reactive Protein (hsCRP)  ELISA

Cat. Num. AAA494066

• Gene Names: CRP; PTX1
• Reactivity: Human
• Synonyms: C-Reactive Protein; C-Reactive Protein (hsCRP) ELISA; hsCRP elisa kit

• Reactivity: Human
• Specificity: The specificity of the hs-CRP ELISA kit was determined by measuring the apparent CRP value of samples spiked with the following compounds.
• Sequence Length: 224

• Samples: Human serum
• Assay Type: Sandwich
• Intra-assay Precision: Three samples were assayed ten times each on the same calibrator curve.
• Inter-assay Precision: Three samples were assayed ten times over a period of four weeks.
• Preparation and Storage: 2 - 8 degree C

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for hsCRP elisa kit

Intended Uses: For the determination of C-Reactive Protem by enzyme immunoassay in human serum.

Principle of the Assay: The principle of the following enzyme immunoassay test follows a typical two-step capture or 'sandwich' type assay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for CRP is immobilized onto the microwell plate and another monoclonal antibody specific for a different region of CRP is conjugated to horse radish peroxidase (HRP). CRP from the sample and calibrators are allowed to bind to the plate, washed, and subsequently incubated with the HRP conjugate. After a second washing step, the enzyme substrate is added. The enzymatic reaction is tenninated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed by the enzymatic reaction is directly proportional to the concentration of CRP in the sample A set of calibrators is used to plot a standard curve from which the amount of CRP in samples and controls can be directly read.

Product Categories/Family for hsCRP elisa kit

Cytokines & Cell Signalling; Research Assays; Assay Categories

NCBI and Uniprot Product Information

• NCBI GI #: 55770842
• NCBI GeneID: 1401
• NCBI Accession #: NP_000558.2
• NCBI GenBank Nucleotide #: NM_000567.2
• Molecular Weight: 10,415 Da
• NCBI Official Full Name: C-reactive protein
• NCBI Official Synonym Full Names: C-reactive protein, pentraxin-related
• NCBI Official Symbol: CRP
• NCBI Official Synonym Symbols: PTX1
• NCBI Protein Information: C-reactive protein; pentraxin 1
• UniProt Protein Name: C-reactive protein
• Protein Family: C-reactive protein
• UniProt Gene Name: CRP
• UniProt Synonym Gene Names: PTX1
• UniProt Entry Name: CRP_HUMAN

NCBI Description

The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. [provided by RefSeq, Sep 2009]

Uniprot Description

CRP: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. Belongs to the pentaxin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q23.2

Cellular Component: extracellular space; growth cone; extracellular region; filopodium

Molecular Function: choline binding; protein binding; protein homodimerization activity; low-density lipoprotein receptor binding; complement component C1q binding; virion binding; low-density lipoprotein binding; cholesterol binding; calcium ion binding

Biological Process: protein polymerization; defense response to Gram-positive bacterium; response to ethanol; wound healing; negative regulation of vasodilation; positive regulation of superoxide release; response to lead ion; acute-phase response; response to hypoxia; opsonization; inflammatory response; complement activation, classical pathway; aging