Mouse Cholinergic Receptor, Muscarinic 3 (CHRM3) ELISA Kit | CHRM3 elisa kit

Mouse Cholinergic Receptor, Muscarinic 3 (CHRM3) ELISA Kit

Cat. Num. AAA2019524

• Gene Names: CHRM3; HM3; PBS; EGBRS
• Reactivity: Mouse
• Synonyms: Cholinergic Receptor; Muscarinic 3 (CHRM3); Mouse Cholinergic Receptor; Muscarinic 3 (CHRM3) ELISA Kit; CHR-M3; HM3; M-AChRM3; Muscarinic Acetylcholine Receptor M3; Muscarinic 3; CHRM3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cholinergic Receptor, Muscarinic 3 (CHRM3).; No significant cross-reactivity or interference between Cholinergic Receptor, Muscarinic 3 (CHRM3) and analogues was observed.
• Sequence Length: 590

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.113ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholinergic Receptor, Muscarinic 3 (CHRM3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholinergic Receptor, Muscarinic 3 (CHRM3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHRM3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholinergic Receptor, Muscarinic 3 (CHRM3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholinergic Receptor, Muscarinic 3 (CHRM3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholinergic Receptor, Muscarinic 3 (CHRM3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholinergic Receptor, Muscarinic 3 (CHRM3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CHRM3 elisa kit

Signal transduction; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 111306506
• NCBI GeneID: 1131
• UniProt Accession #: P20309; Q0VAJ8; Q4QRI3; Q5VXY2; Q9HB60
• Molecular Weight: 66,128 Da
• NCBI Official Full Name: Cholinergic receptor, muscarinic 3
• NCBI Official Synonym Full Names: cholinergic receptor, muscarinic 3
• NCBI Official Symbol: CHRM3
• NCBI Official Synonym Symbols: HM3; PBS; EGBRS
• NCBI Protein Information: muscarinic acetylcholine receptor M3
• UniProt Protein Name: Muscarinic acetylcholine receptor M3
• Protein Family: Muscarinic acetylcholine receptor
• UniProt Gene Name: CHRM3
• UniProt Entry Name: ACM3_HUMAN

NCBI Description

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. [provided by RefSeq, Jul 2008]

Uniprot Description

mAChR M3: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. Defects in CHRM3 are the cause of Eagle-Barrett syndrome (EGBRS). EGBRS is a syndrome characterized by thin abdominal musculature with overlying lax skin, cryptorchism, megacystis with disorganized detrusor muscle, and urinary tract abnormalities. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.

Protein type: Membrane protein, integral; GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: asymmetric synapse; postsynaptic membrane; basolateral plasma membrane; integral to plasma membrane; dendrite; plasma membrane; nerve terminal; cell junction

Molecular Function: protein binding; receptor activity; drug binding; acetylcholine binding; phosphoinositide phospholipase C activity; G-protein coupled acetylcholine receptor activity

Biological Process: nervous system development; cell proliferation; G-protein coupled receptor protein signaling pathway; acetylcholine receptor signaling, muscarinic pathway; smooth muscle contraction; regulation of vascular smooth muscle contraction; energy reserve metabolic process; protein modification process; positive regulation of smooth muscle contraction; signal transduction; regulation of insulin secretion; saliva secretion

Disease: Abdominal Muscles, Absence Of, With Urinary Tract Abnormality And Cryptorchidism