Rabbit anti-Human NPHP4 Polyclonal Antibody | anti-NPHP4 antibody

Rabbit Polyclonal (IgG) to Human NPHP4

Cat. Num. AAA247731

• Gene Names: NPHP4; POC10; SLSN4
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: NPHP4; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human NPHP4; Anti-NPHP4 Antibody (aa877-926) IHC-plus; Human NPHP4; anti-NPHP4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NPHP4 Antibody detects endogenous levels of total NPHP4 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1426

• Applicable Applications for anti-NPHP4 antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA (1:40000), IHC-P (5 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human NPHP4 (aa877-926).
• Immunogen: NPHP4 antibody was raised against synthetic peptide from human NPHP4 (aa877-926).
• Antigen Modification: aa877-926
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-NPHP4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.)

Related Product Information for anti-NPHP4 antibody

This gene encodes a protein involved in renal tubular development and function. This protein interacts with nephrocystin, and belongs to a multifunctional complex that is localized to actin- and microtubule-based structures. Mutations in this gene are associated with nephronophthisis type 4, a renal disease, and with Senior-Loken syndrome type 4, a combination of nephronophthisis and retinitis pigmentosa.

NCBI and Uniprot Product Information

• NCBI GI #: 23510323
• NCBI GeneID: 261734
• NCBI Accession #: NP_055917.1
• NCBI GenBank Nucleotide #: NM_015102.4
• UniProt Accession #: O75161; Q8IWC0
• Molecular Weight: 99,953 Da
• NCBI Official Full Name: nephrocystin-4 isoform a
• NCBI Official Synonym Full Names: nephronophthisis 4
• NCBI Official Symbol: NPHP4
• NCBI Official Synonym Symbols: POC10; SLSN4
• NCBI Protein Information: nephrocystin-4; nephroretinin; POC10 centriolar protein homolog
• UniProt Protein Name: Nephrocystin-4
• Protein Family: Nephrocystin
• UniProt Gene Name: NPHP4
• UniProt Synonym Gene Names: KIAA0673
• UniProt Entry Name: NPHP4_HUMAN

NCBI Description

This gene encodes a protein involved in renal tubular development and function. This protein interacts with nephrocystin, and belongs to a multifunctional complex that is localized to actin- and microtubule-based structures. Mutations in this gene are associated with nephronophthisis type 4, a renal disease, and with Senior-Loken syndrome type 4, a combination of nephronophthisis and retinitis pigmentosa. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Uniprot Description

NPHP4: Involved in the organization of apical junctions in kidney cells together with NPHP1 and RPGRIP1L/NPHP8. Does not seem to be strictly required for ciliogenesis. Defects in NPHP4 are the cause of nephronophthisis type 4 (NPHP4); also known as familial juvenile nephronophthisis 4. NPHP4 is an autosomal recessive inherited disease resulting in end-stage renal disease at age ranging between 6 and 35 years. It is a progressive tubulo-interstitial kidney disorder characterized by polydipsia, polyuria, anemia and growth retardation. The most prominent histological features are modifications of the tubules with thickening of the basement membrane, interstitial fibrosis and, in the advanced stages, medullary cysts. Ciliary dysfunction leads to a broad spectrum of disorders, collectively termed ciliopathies. Overlapping clinical features include retinal degeneration, renal cystic disease, skeletal abnormalities, fibrosis of various organ, and a complex range of anatomical and functional defects of the central and peripheral nervous system. The ciliopathy range of diseases includes Meckel-Gruber syndrome, Bardet-Biedl syndrome, Joubert syndrome, nephronophtisis, Senior-Loken syndrome, and Jeune asphyxiating thoracic dystrophy among others. Single-locus allelism is insufficient to explain the variable penetrance and expressivity of such disorders, leading to the suggestion that variations across multiple sites of the ciliary proteome, including NPHP4, influence the clinical outcome. Defects in NPHP4 are the cause of Senior-Loken syndrome type 4 (SLSN4). SLSN is a renal-retinal disorder characterized by progressive wasting of the filtering unit of the kidney, with or without medullary cystic renal disease, and progressive eye disease. Typically this disorder becomes apparent during the first year of life. Belongs to the NPHP4 family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: centrosome; tight junction; membrane; intercellular junction; cytosol; photoreceptor connecting cilium

Molecular Function: protein binding; structural molecule activity

Biological Process: sperm motility; cell-cell adhesion; retina development in camera-type eye; organelle organization and biogenesis; photoreceptor cell maintenance; actin cytoskeleton organization and biogenesis; signal transduction; visual behavior

Disease: Nephronophthisis 4; Senior-loken Syndrome 4