Rabbit EMR3 Polyclonal Antibody | anti-EMR3 antibody

Rabbit Polyclonal to Human EMR3

Cat. Num. AAA240942

• Reactivity: Gorilla, Human, Monkey; Predicted Reactivity: Orangutan, Gibbon (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: EMR3; Polyclonal Antibody; Rabbit Polyclonal to Human EMR3; Anti-EMR3 Antibody (C-Terminus) IHC-plus; EGF-like module receptor 3; Human EMR3; anti-EMR3 antibody

• Host: Rabbit
• Reactivity: Gorilla, Human, Monkey; Predicted Reactivity: Orangutan, Gibbon (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human EMR3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 652

• Applicable Applications for anti-EMR3 antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA, IHC-P (2.5 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic 18 amino acid peptide from C-terminal cytoplasmic domain of human EMR3. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%); Orangutan, Gibbon (94%); Elephant (89%).
• Immunogen Type: Synthetic peptide
• Immunogen: EMR3 antibody was raised against synthetic 18 amino acid peptide from C-terminal cytoplasmic domain of human EMR3. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%); Orangutan, Gibbon (94%); Elephant (89%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-EMR3 antibody IHC of human macrophage. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-EMR3 antibody

EMR3 is an Orphan-B GPCR with an unknown ligand. Two variants are produced by alternative splicing. One form encodes a 652-amino acid GPCR consisting of two EGF-like domains and a mucin stalk, while the other is a truncated soluble form containing only two EGF-like domains. EMR3 and EMR2 share a high degree of sequence identity in the transmembrane domains.

Product Categories/Family for anti-EMR3 antibody

Family: GPCR; Subfamily: Orphan-B

NCBI and Uniprot Product Information

• NCBI GI #: 133930786
• NCBI GeneID: 84658
• NCBI Accession #: NP_115960.2
• NCBI GenBank Nucleotide #: NM_032571.4
• UniProt Accession #: Q9BY15
• Molecular Weight: 6,996 Da
• NCBI Official Full Name: EGF-like module-containing mucin-like hormone receptor-like 3 isoform 1
• NCBI Official Synonym Full Names: egf-like module containing, mucin-like, hormone receptor-like 3
• NCBI Official Symbol: EMR3
• NCBI Protein Information: EGF-like module-containing mucin-like hormone receptor-like 3; EGF-like module receptor 3; egf-like module-containing mucin-like receptor 3
• UniProt Protein Name: EGF-like module-containing mucin-like hormone receptor-like 3
• UniProt Gene Name: EMR3
• UniProt Entry Name: EMR3_HUMAN

NCBI Description

This gene encodes a member of the class B seven-span transmembrane (TM7) receptor family expressed predominantly by cells of the immune system. Family members are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor (EGF)-like domains coupled to a TM7 domain via a mucin-like spacer domain. This gene is closely linked to the gene encoding egf-like molecule containing mucin-like hormone receptor 2 on chromosome 19. This protein may play a role in myeloid-myeloid interactions during immune and inflammatory responses. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2014]

Uniprot Description

EMR3: Receptor probably involved in myeloid interactions during immune and inflammatory responses. A ligand for the soluble form of this receptor is present at the surface of momocytes- derived macrophages and activated neutrophils. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 2

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: extracellular space; integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; calcium ion binding

Biological Process: G-protein coupled receptor protein signaling pathway