Human CCL8/MCP-2 ELISA Kit | CCL8 elisa kit

Human CCL8/MCP-2 PicoKine ELISA Kit

Cat. Num. AAA176549

• Gene Names: CCL8; HC14; MCP2; MCP-2; SCYA8; SCYA10
• Reactivity: Human
• Synonyms: CCL8/MCP-2; Human CCL8/MCP-2 PicoKine ELISA Kit; C-C motif chemokine 8; chemokine (C-C motif) ligand 8; C C motif chemokine 8; CCL 8; Ccl8; CCL8_HUMAN; Chemokine (C C motif) ligand 8; Chemokine ligand 8; HC14; MCP 2; MCP-2; MCP-2(6-76); Monocyte chemoattractant protein 2; Monocyte chemotactic protein 2; SCYA10; SCYA8; Small inducible cytokine A8; Small inducible cytokine subfamily A (Cys Cys) member 8; Small-inducible cytokine A8; CCL8 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human CCL8
• Sequence Length: 99

• Samples: Cell Culture Supernates, Cell Lysates, Serum And Plasma (Heparin, Edta)
• Immunogen: E Coli, A27-M96
• Reactivity Note: Human. There is no detectable cross-reactivity with other relevant proteins
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCL8 elisa kit

Principle of the Assay: The Picokine Human CCL20 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CCL20 with a 96-well strip plate that is pre-coated with antibody specific for CCL20. The detection antibody is a biotinylated antibody specific for CCL20. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human CCL20 with immunogen: Expression system for standard: E.coli; Immunogen sequence: A27-M96. The kit is analytically validated with ready to use reagents. To measure Human CCL20, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human CCL20 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CCL20 in the sample!!Background/Introduction: Macrophage Inflammatory Protein 3alpha(MIP3alpha), also called Chemokine, cc motif, ligand 20(CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly upregulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.

NCBI and Uniprot Product Information

• NCBI GI #: 2506280
• NCBI GeneID: 6355
• UniProt Accession #: P80075; P78388; A0AV77
• Molecular Weight: 11,246 Da
• NCBI Official Full Name: C-C motif chemokine 8
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 8
• NCBI Official Symbol: CCL8
• NCBI Official Synonym Symbols: HC14; MCP2; MCP-2; SCYA8; SCYA10
• NCBI Protein Information: C-C motif chemokine 8; small-inducible cytokine A8; monocyte chemotactic protein 2; monocyte chemoattractant protein 2; small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2)
• UniProt Protein Name: C-C motif chemokine 8
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL8
• UniProt Synonym Gene Names: MCP2; SCYA10; SCYA8; MCP-2
• UniProt Entry Name: CCL8_HUMAN

NCBI Description

This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL8: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Chemokine; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: extracellular space

Molecular Function: heparin binding; chemokine activity; phospholipase activator activity; protein kinase activity

Biological Process: positive regulation of catalytic activity; cellular calcium ion homeostasis; exocytosis; cell-cell signaling; calcium ion transport; response to virus; immune response; chemotaxis; inflammatory response; signal transduction; protein amino acid phosphorylation