Human Tubulin beta2A Chain ELISA Kit | TUBB2A elisa kit

Human Tubulin beta2A Chain ELISA Kit

Cat. Num. AAA7254761

• Gene Names: TUBB2A; TUBB; TUBB2; CDCBM5
• Reactivity: Human
• Synonyms: Tubulin beta2A Chain; Human Tubulin beta2A Chain ELISA Kit; TUBB2A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TUBB2A. No significant cross-reactivity or interference between TUBB2A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TUBB2A and all the analogues, therefore, cross reaction may still exist in some cases.
• Sequence Length: 445

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store at 2-8 degree C.; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TUBB2A elisa kit

Intended Uses: This TUBB2A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TUBB2A. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: TUBB2A ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TUBB2A antibody and an TUBB2A-HRP conjugate. The assay sample and buffer are incubated together with TUBB2A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TUBB2A concentration since TUBB2A from samples and TUBB2A-HRP conjugate compete for the anti-TUBB2A antibody binding site. Since the number of sites is limited, as more sites are occupied by TUBB2A from the sample, fewer sites are left to bind TUBB2A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TUBB2A concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4507729
• NCBI GeneID: 7280
• NCBI Accession #: NP_001060.1
• NCBI GenBank Nucleotide #: NM_001069.3
• UniProt Accession #: Q13885; Q6FGZ8; Q8IWR2
• Molecular Weight: 49,907 Da
• NCBI Official Full Name: tubulin beta-2A chain isoform 1
• NCBI Official Synonym Full Names: tubulin beta 2A class IIa
• NCBI Official Symbol: TUBB2A
• NCBI Official Synonym Symbols: TUBB; TUBB2; CDCBM5
• NCBI Protein Information: tubulin beta-2A chain
• UniProt Protein Name: Tubulin beta-2A chain
• Protein Family: Tubulin
• UniProt Gene Name: TUBB2A
• UniProt Synonym Gene Names: TUBB2

NCBI Description

Microtubules, key participants in processes such as mitosis and intracellular transport, are composed of heterodimers of alpha- and beta-tubulins. The protein encoded by this gene is a beta-tubulin. Defects in this gene are associated with complex cortical dysplasia with other brain malformations-5. Two transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2015]

Uniprot Description

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain ().