Mouse Calreticulin-3 (CALR3) ELISA Kit | CALR3 elisa kit

Mouse Calreticulin-3 (CALR3) ELISA Kit

Cat. Num. AAA7239797

• Gene Names: CALR3; CRT2; CT93; CMH19
• Reactivity: Mouse
• Synonyms: Calreticulin-3 (CALR3); Mouse Calreticulin-3 (CALR3) ELISA Kit; CALR3 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL.
• Assay Type: Competitive
• Intended Uses: This CALR3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CALR3. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CALR3 elisa kit

Principle of the Assay: CALR3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CALR3 antibody and an CALR3-HRP conjugate. The assay sample and buffer are incubated together with CALR3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CALR3 concentration since CALR3 from samples and CALR3-HRP conjugate compete for the anti-CALR3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CALR3 from the sample, fewer sites are left to bind CALR3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CALR3 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CALR3 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 187608348
• NCBI GeneID: 125972
• NCBI Accession #: NP_659483.2
• NCBI GenBank Nucleotide #: NM_145046.4
• Molecular Weight: 44,996 Da
• NCBI Official Full Name: calreticulin-3
• NCBI Official Synonym Full Names: calreticulin 3
• NCBI Official Symbol: CALR3
• NCBI Official Synonym Symbols: CRT2; CT93; CMH19
• NCBI Protein Information: calreticulin-3; calsperin; calreticulin-2; cancer/testis antigen 93
• UniProt Protein Name: Calreticulin-3
• Protein Family: Calreticulin
• UniProt Gene Name: CALR3
• UniProt Synonym Gene Names: CRT2
• UniProt Entry Name: CALR3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the calreticulin family, members of which are calcium-binding chaperones localized mainly in the endoplasmic reticulum. This protein is also localized to the endoplasmic reticulum lumen, however, its capacity for calcium-binding may be absent or much lower than other family members. This gene is specifically expressed in the testis, and may be required for sperm fertility. Mutation in this gene has been associated with familial hypertrophic cardiomyopathy. [provided by RefSeq, Dec 2011]

Uniprot Description

calreticulin 3: During spermatogenesis, may act as a lectin-independent chaperone for specific client proteins such as ADAM3. Required for sperm fertility. CALR3 capacity for calcium- binding may be absent or much lower than that of CALR. Defects in CALR3 are the cause of familial hypertrophic cardiomyopathy type 19 (CMH19). CMH19 is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Belongs to the calreticulin family.

Protein type: Cancer Testis Antigen (CTA); Chaperone

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: endoplasmic reticulum lumen

Molecular Function: unfolded protein binding; calcium ion binding; carbohydrate binding

Biological Process: protein folding; spermatogenesis; cell differentiation

Disease: Cardiomyopathy, Familial Hypertrophic, 19