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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Human ATP Binding Cassette Transporter E1 Polyclonal Antibody | anti-ABCE1 antibody

Polyclonal Antibody to ATP Binding Cassette Transporter E1 (ABCE1)

Gene Names
ABCE1; RLI; OABP; RLI1; ABC38; RNS4I; RNASEL1; RNASELI
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
ATP Binding Cassette Transporter E1; Polyclonal Antibody; Polyclonal Antibody to ATP Binding Cassette Transporter E1 (ABCE1); ABC-E1; RLI; OABP; RNASEL1; RNASELI; RNS4I; HuHP68; 2'-5'-oligoadenylate-binding protein; RNase L inhibitor; Ribonuclease 4 inhibitor; anti-ABCE1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against ABCE1. It has been selected for its ability to recognize ABCE1 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-ABCE1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Fragment
ABCE1 (Ile389~Asn556)
Conjugate
No Conjugate
Cross Reactivity
Human
Immunoglobulin Type
IgG
Quality Control
Content: The quality control contains recombinant ABCE1 (Ile389~Asn556) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2072129
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded rectum tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded rectum tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
67,314 Da
NCBI Official Full Name
ATP-binding cassette sub-family E member 1
NCBI Official Synonym Full Names
ATP binding cassette subfamily E member 1
NCBI Official Symbol
ABCE1
NCBI Official Synonym Symbols
RLI; OABP; RLI1; ABC38; RNS4I; RNASEL1; RNASELI
NCBI Protein Information
ATP-binding cassette sub-family E member 1
UniProt Protein Name
ATP-binding cassette sub-family E member 1
Protein Family
UniProt Gene Name
ABCE1
UniProt Synonym Gene Names
RLI; RNASEL1; RNASELI; RNS4I; RNS4I

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the OABP subfamily. Alternatively referred to as the RNase L inhibitor, this protein functions to block the activity of ribonuclease L. Activation of ribonuclease L leads to inhibition of protein synthesis in the 2-5A/RNase L system, the central pathway for viral interferon action. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCE1: Antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly. Belongs to the ABC transporter superfamily. ABCE family.

Chromosomal Location of Human Ortholog: 4q31.21

Cellular Component: cytoplasm; cytosol; eukaryotic translation initiation factor 3 complex; membrane; mitochondrial matrix; mitochondrion

Molecular Function: ATP binding; ATPase activity; iron ion binding; protein binding; ribosomal small subunit binding

Biological Process: ribosomal subunit export from nucleus; translational initiation; translational termination; viral process

Research Articles on ABCE1

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Product Notes

The ABCE1 abce1 (Catalog #AAA2033927) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to ATP Binding Cassette Transporter E1 (ABCE1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATP Binding Cassette Transporter E1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-1:5000. Researchers should empirically determine the suitability of the ABCE1 abce1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP Binding Cassette Transporter E1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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