Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Beta-1,3-N-acetylglucosaminyltransferase manic fringe (MFNG) Standard Curve.)
Mouse Beta-1,3-N-acetylglucosaminyltransferase Manic Fringe (MFNG) ELISA Kit | MFNG elisa kit
Mouse Beta-1,3-N-acetylglucosaminyltransferase Manic Fringe (MFNG) ELISA Kit
Gene Names
Mfng; AW546563
Reactivity
Mouse
Synonyms
Beta-1; 3-N-acetylglucosaminyltransferase Manic Fringe (MFNG); Mouse Beta-1; 3-N-acetylglucosaminyltransferase Manic Fringe (MFNG) ELISA Kit; MFNG; CTA-390B3.1; beta-1; 3-N-acetylglucosaminyltransferase manic fringe; MFNG elisa kit
Reactivity
Mouse
Specificity
Mouse Beta-1,3-N-acetylglucosaminyltransferase manic fringe (MFNG) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MFNG. No significant cross-reactivity or interference between Mouse MFNG and analogues was observed.
Sequence Length
321
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Beta-1,3-N-acetylglucosaminyltransferase manic fringe (MFNG) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Beta-1,3-N-acetylglucosaminyltransferase manic fringe (MFNG) Standard Curve.)
Related Product Information for MFNG elisa kit
Description: This Mouse Beta-1,3-N-acetylglucosaminyltransferase manic fringe (MFNG) ELISA Kit employs a two-site sandwich ELISA to quantitate MFNG in samples. An antibody specific for MFNG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MFNG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MFNG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MFNG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MFNG is a member of the fringe gene family which also includes radical and lunatic fringe genes. They all encode evolutionarily conserved secreted proteins that act in the Notch receptor pathway to demarcate boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling.
Background: MFNG is a member of the fringe gene family which also includes radical and lunatic fringe genes. They all encode evolutionarily conserved secreted proteins that act in the Notch receptor pathway to demarcate boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
beta-1,3-N-acetylglucosaminyltransferase manic fringe
NCBI Official Synonym Full Names
MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
NCBI Official Symbol
Mfng
NCBI Official Synonym Symbols
AW546563
NCBI Protein Information
beta-1,3-N-acetylglucosaminyltransferase manic fringe
UniProt Protein Name
Beta-1,3-N-acetylglucosaminyltransferase manic fringe
Protein Family
UniProt Gene Name
Mfng
UniProt Entry Name
MFNG_MOUSE
Research Articles on MFNG
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Product Notes
The Mouse MFNG mfng (Catalog #AAA9717648) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717648 ELISA Kit recognizes Mouse MFNG. It is sometimes possible for the material contained within the vial of "Beta-1,3-N-acetylglucosaminyltransferase Manic Fringe (MFNG), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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