Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (MGAT1) Standard Curve.)
Human Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (MGAT1) ELISA Kit | MGAT1 elisa kit
Human Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (MGAT1) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (MGAT1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (MGAT1) Standard Curve.)
Background: There are over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I, is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The exon includes most of the 5-prime untranslated region, the complete coding sequence, and the complete 3-prime untranslated region. Southern blot analysis indicated that the gene exists in single copy in the human genome, and study of human-hamster somatic cell hybrids indicated that the gene is located on chromosome 5. GlcNAc-T I is expressed as 2 mRNAs of 3.1 and 2.7 to 3.0 kb in all tissues tested, although only the 3.1-kb mRNA is seen in brain, and expression levels are low.
NCBI and Uniprot Product Information
NCBI Description
There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
MGAT1: Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans. Belongs to the glycosyltransferase 13 family.
Protein type: Glycan Metabolism - N-glycan biosynthesis; EC 2.4.1.101; Transferase; Membrane protein, integral
Chromosomal Location of Human Ortholog: 5q35
Cellular Component: Golgi membrane; membrane; integral to membrane; vesicle
Molecular Function: acetylglucosaminyltransferase activity; metal ion binding; alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity
Biological Process: cellular protein metabolic process; in utero embryonic development; carbohydrate metabolic process; protein amino acid glycosylation; protein amino acid N-linked glycosylation via asparagine; UDP-N-acetylglucosamine catabolic process; post-translational protein modification
Research Articles on MGAT1
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Product Notes
The Human MGAT1 mgat1 (Catalog #AAA9716030) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716030 ELISA Kit recognizes Human MGAT1. It is sometimes possible for the material contained within the vial of "Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (MGAT1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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