Mouse BAD ELISA Kit | BAD elisa kit

Mouse BAD (Bcl-2 Associated Death Promoter) ELISA Kit

Cat. Num. AAA2501563

• Gene Names: BAD; BBC2; BCL2L8
• Reactivity: Mouse
• Synonyms: BAD; Mouse BAD (Bcl-2 Associated Death Promoter) ELISA Kit; BAD elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes natural and recombinantMouseBAD. No significant cross-reactivity or interference between MouseBAD and analogues was observed.
• Sequence Length: 168

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BAD elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of MouseBADconcentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BAD. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for BADand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BAD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of BAD.You can calculate the concentration of BADin the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 14670388
• NCBI GeneID: 572
• NCBI Accession #: NP_116784.1
• NCBI GenBank Nucleotide #: NM_032989.2
• UniProt Accession #: Q92934; O14803; Q6FH21
• Molecular Weight: 18,392 Da
• NCBI Official Full Name: bcl2 antagonist of cell death
• NCBI Official Synonym Full Names: BCL2-associated agonist of cell death
• NCBI Official Symbol: BAD
• NCBI Official Synonym Symbols: BBC2; BCL2L8
• NCBI Protein Information: bcl2-associated agonist of cell death; BCL-X/BCL-2 binding protein; BCL2-antagonist of cell death protein; BCL2-binding component 6; BCL2-binding protein; bcl-2-binding component 6; bcl-2-like protein 8; bcl-XL/Bcl-2-associated death promoter; bcl2 antagonist of cell death; bcl2-L-8
• UniProt Protein Name: Bcl2-associated agonist of cell death
• Protein Family: Bcl2-associated agonist of cell death
• UniProt Gene Name: BAD
• UniProt Synonym Gene Names: BBC6; BCL2L8; BAD; Bcl2-L-8
• UniProt Entry Name: BAD_HUMAN

NCBI Description

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008]

Uniprot Description

BAD: a proapoptotic member of the Bcl-2 family. Displaces Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad inducing the phosphorylation of Bad by Akt and p90RSK. 14-3-3 proteins bind phosphorylated Bad, inhibiting its binding to Bcl-2 and Bcl-xL. Phosphorylation by mitochondria-anchored PKA in the BH3 domain can block the dimerization of Bad and Bcl-xL.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol

Molecular Function: protein kinase B binding; protein binding; protein heterodimerization activity; phospholipid binding; caspase activator activity; lipid binding; protein kinase binding; protein phosphatase 2B binding

Biological Process: response to oleate; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; positive regulation of proteolysis; response to glucocorticoid stimulus; positive regulation of caspase activity; glucose homeostasis; cellular process regulating host cell cycle in response to virus; response to estradiol stimulus; positive regulation of apoptosis by virus; response to glucose stimulus; pore complex biogenesis; positive regulation of autophagy; positive regulation of glucokinase activity; caspase activation; epidermal growth factor receptor signaling pathway; response to drug; release of cytochrome c from mitochondria; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; cytokine and chemokine mediated signaling pathway; suppression by virus of host apoptosis; ADP metabolic process; positive regulation of insulin secretion; response to testosterone stimulus; response to amino acid stimulus; ATP metabolic process; glucose catabolic process; response to ethanol; induction of apoptosis via death domain receptors; response to hydrogen peroxide; positive regulation of T cell differentiation; DNA damage response, signal transduction resulting in induction of apoptosis; innate immune response; positive regulation of B cell differentiation; regulation of mitochondrial membrane permeability; response to progesterone stimulus; response to calcium ion; positive regulation of epithelial cell proliferation