Rabbit anti-Human MLLT1 Polyclonal Antibody | anti-MLLT1 antibody

MLLT1, CT (MLLT1, ENL, LTG19, YEATS1, Protein ENL, YEATS domain-containing protein 1) (Biotin)

Cat. Num. AAA6321154

• Gene Names: MLLT1; ENL; LTG19; YEATS1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: MLLT1; Polyclonal Antibody; CT (MLLT1; ENL; LTG19; YEATS1; Protein ENL; YEATS domain-containing protein 1) (Biotin); YEATS domain-containing protein 1; anti-MLLT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MLLT1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MLLT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 530~559 amino acids from the C-terminal region of human MLLT1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-MLLT1 Pab in A257 cell line lysate (35ug/lane). MLLT1(arrow) was detected using the purified Pab.)

Related Product Information for anti-MLLT1 antibody

Chromosome band 11q23 is the site of translocations in myeloid and lymphoid acute leukemias, pediatric leukemias, and treatment-induced secondary acute myelogenous leukemia. The translocation breakpoints cluster in a restricted region of the HRX gene resulting in chimeric genes that encode an N-terminal portion of Hrx fused to various partner proteins. Myeloid/lymphoid or mixed-lineage leukemia translocated to 1 (MLLT1) is a nuclear protein with transcriptional transactivation properties that is fused to Hrx in t(11;19) leukemias. The minimal MLLT1 sequence required for transcription activation was narrowed to the C-terminal 90 amino acids.

Product Categories/Family for anti-MLLT1 antibody

Antibodies; Transcription Factors

References

Nie, Z., et al., Mol. Cell. Biol. 23(8):2942-2952 (2003). Lavau, C., et al., Proc. Natl. Acad. Sci. U.S.A. 97(20):10984-10989 (2000). Thirman, M.J., et al., Proc. Natl. Acad. Sci. U.S.A. 91(25):12110-12114 (1994). Rubnitz, J.E., et al., Blood 84(6):1747-1752 (1994). Yamamoto, K., et al., Oncogene 8(10):2617-2625 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 21361272
• NCBI GeneID: 4298
• NCBI Accession #: NP_005925
• NCBI GenBank Nucleotide #: NM_005934
• UniProt Accession #: Q03111
• Molecular Weight: 62kDa
• NCBI Official Full Name: protein ENL
• NCBI Official Synonym Full Names: MLLT1 super elongation complex subunit
• NCBI Official Symbol: MLLT1
• NCBI Official Synonym Symbols: ENL; LTG19; YEATS1
• NCBI Protein Information: protein ENL
• UniProt Protein Name: Protein ENL
• Protein Family: Protein
• UniProt Gene Name: MLLT1
• UniProt Synonym Gene Names: ENL; LTG19; YEATS1
• UniProt Entry Name: ENL_HUMAN

Uniprot Description

MLLT1: a transcriptional regulator that can activate transcription from synthetic reporter genes in both lymphoid and myeloid cells. A chromosomal aberration involving MLLT1 is associated with acute leukemias.

Protein type: Oncoprotein; Transcription regulation

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; transcription elongation factor complex; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; DNA binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; negative regulation of protein kinase activity