Rat Histidine Triad Nucleotide Binding Protein 1 (HINT1) ELISA Kit | HINT1 elisa kit

Rat Histidine Triad Nucleotide Binding Protein 1 (HINT1) ELISA Kit

Cat. Num. AAA2706539

• Gene Names: HINT1; HINT; NMAN; PKCI-1; PRKCNH1
• Reactivity: Rat
• Synonyms: Histidine Triad Nucleotide Binding Protein 1 (HINT1); Rat Histidine Triad Nucleotide Binding Protein 1 (HINT1) ELISA Kit; HINT; PKCI1; PRKCNH1; Adenosine 5'-monophosphoramidase; Protein kinase C inhibitor 1; Protein kinase C-interacting protein 1; HINT1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Histidine Triad Nucleotide Binding Protein 1 (HINT1).; No significant cross-reactivity or interference between Histidine Triad Nucleotide Binding Protein 1 (HINT1) and analogues was observed.
• Sequence Length: 126

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.113ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histidine Triad Nucleotide Binding Protein 1 (HINT1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histidine Triad Nucleotide Binding Protein 1 (HINT1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HINT1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histidine Triad Nucleotide Binding Protein 1 (HINT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histidine Triad Nucleotide Binding Protein 1 (HINT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histidine Triad Nucleotide Binding Protein 1 (HINT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histidine Triad Nucleotide Binding Protein 1 (HINT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119582780
• NCBI GeneID: 3094
• Molecular Weight: 13,802 Da
• NCBI Official Full Name: histidine triad nucleotide binding protein 1
• NCBI Official Synonym Full Names: histidine triad nucleotide binding protein 1
• NCBI Official Symbol: HINT1
• NCBI Official Synonym Symbols: HINT; NMAN; PKCI-1; PRKCNH1
• NCBI Protein Information: histidine triad nucleotide-binding protein 1
• UniProt Protein Name: Histidine triad nucleotide-binding protein 1
• Protein Family: Adenylylsulfatase
• UniProt Gene Name: HINT1
• UniProt Synonym Gene Names: HINT; PKCI1; PRKCNH1; PKCI-1

NCBI Description

This gene encodes a protein that hydrolyzes purine nucleotide phosphoramidates substrates, including AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif. This gene is considered a tumor suppressor gene. In addition, mutations in this gene can cause autosomal recessive neuromyotonia and axonal neuropathy. There are several related pseudogenes on chromosome 7. Several transcript variants have been observed. [provided by RefSeq, Dec 2015]

Uniprot Description

HINT1: Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha- acetyl lysine methyl ester and AMP-NH2. Belongs to the HINT family.

Protein type: Cytoskeletal; EC 3.-.-.-; Hydrolase

Chromosomal Location of Human Ortholog: 5q23.3

Cellular Component: cytoskeleton; cytosol; extracellular exosome; histone deacetylase complex; nucleus; plasma membrane

Molecular Function: hydrolase activity; nucleotide binding; protein kinase C binding

Biological Process: intrinsic apoptotic signaling pathway by p53 class mediator; positive regulation of calcium-mediated signaling; purine ribonucleotide catabolic process; regulation of transcription, DNA-templated; signal transduction; transcription, DNA-dependent

Disease: Neuromyotonia And Axonal Neuropathy, Autosomal Recessive