PDE9A recombinant protein

Recombinant Human PDE9A Protein (His & GST tag)

Cat. Num. AAA2545680

• Gene Names: PDE9A; HSPDE9A2
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: PDE9A; Recombinant Human PDE9A Protein (His & GST tag); FLJ90181; HSPDE9A2; PDE9A recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 5mM GSH, pH 7.4, 10% gly
• Sequence Length: 533

• Application Notes: The recombinant human PDE9A consisting of 770 amino acids and has a calculated molecular mass of 89.5 kDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for PDE9A recombinant protein

Background: High affinity cGMP-specific 3', 5'-cyclic phosphodiesterase 9A, also known as PDE9A, is a member of the cyclic nucleotide phosphodiesterase family and PDE9 subfamily. PDE9A is expressed in all tissues examined (testis, brain, small intestine, skeletal muscle, heart, lung, thymus, spleen, placenta, kidney, liver, pancreas, ovary and prostate) except blood. Highest levels of PDE9A is in brain, heart, kidney, spleen, prostate and colon. Isoform PDE9A12 is found in prostate. PDE9A mRNA is widely distributed throughout the rat and mouse brain, with the highest expression observed in cerebellar Purkinje cells. PDE9A is the only cGMP-specific PDE with significant expression in the forebrain, and as such is likely to play an important role in NO-cGMP signaling. PDE9A is highly conserved between species and is widely distributed throughout the rodent brain. PDE9A is probably involved in maintenance of low cGMP levels in cells and might play an important role in a variety of brain functions involving cGMP-mediated signal transduction. PDE9A hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. PDE9A represents a novel drug target worthy of further study.

Description: A DNA sequence encoding the full length of human PDE9A (O76083-2) (Met 1-Ala 533) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 48762718
• NCBI GeneID: 5152
• NCBI Accession #: NP_001001567.1
• NCBI GenBank Nucleotide #: NM_001001567.1
• UniProt Accession #: O76083; O75490; O75491; O95225; Q53Y40; Q5QD39; Q86SF7; Q86SI6; B2RBI5; B4DFI5; D3DSJ8; D3DSJ9
• Molecular Weight: 56,554 Da
• NCBI Official Full Name: high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A isoform b
• NCBI Official Synonym Full Names: phosphodiesterase 9A
• NCBI Official Symbol: PDE9A
• NCBI Official Synonym Symbols: HSPDE9A2
• NCBI Protein Information: high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A
• UniProt Protein Name: High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A
• Protein Family: High affinity cGMP-specific 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE9A

NCBI Description

The protein encoded by this gene catalyzes the hydrolysis of cAMP and cGMP to their corresponding monophosphates. The encoded protein plays a role in signal transduction by regulating the intracellular concentration of these cyclic nucleotides. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Specifically hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. Highly specific: compared to other members of the cyclic nucleotide phosphodiesterase family, has the highest affinity and selectivity for cGMP (PubMed:9624146, PubMed:18757755, PubMed:21483814). Specifically regulates natriuretic-peptide-dependent cGMP signaling in heart, acting as a regulator of cardiac hypertrophy in myocytes and muscle. Does not regulate nitric oxide-dependent cGMP in heart (PubMed:25799991). Additional experiments are required to confirm whether its ability to hydrolyze natriuretic-peptide-dependent cGMP is specific to heart or is a general feature of the protein (Probable). In brain, involved in cognitive function, such as learning and long-term memory ().