Mouse ApoA1 ELISA Kit | ApoA1 elisa kit

Mouse ApoA1 (Apolipoprotein A1) ELISA Kit

Cat. Num. AAA2516162

• Reactivity: Mouse
• Synonyms: ApoA1; Mouse ApoA1 (Apolipoprotein A1) ELISA Kit; ApoA1 elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes natural and recombinant Mouse ApoA1. No significant cross-reactivity or interference between Mouse ApoA1 and analogues was observed.
• Sequence Length: 267

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 7.813-500ng/mL
• Sensitivity: Min: 4.688ng/mL; Max: 500ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ApoA1 elisa kit

Intended Uses||This ELISA kit applies to the in vitro quantitative determination of Mouse ApoA1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ApoA1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ApoA1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ApoA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ApoA1. You can calculate the concentration of ApoA1 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557321
• NCBI GeneID: 335
• NCBI Accession #: NP_000030.1
• NCBI GenBank Nucleotide #: NM_000039.1
• Molecular Weight: 30,778 Da
• NCBI Official Full Name: apolipoprotein A-I preproprotein
• NCBI Official Synonym Full Names: apolipoprotein A-I
• NCBI Official Symbol: APOA1
• NCBI Protein Information: apolipoprotein A-I; apo-AI
• UniProt Protein Name: Apolipoprotein A-I
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA1
• UniProt Synonym Gene Names: Apo-AI; ApoA-I; ProapoA-I
• UniProt Entry Name: APOA1_HUMAN

NCBI Description

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq, Jul 2008]

Uniprot Description

APOA1: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Motility/polarity/chemotaxis; Lipid-binding; Cell development/differentiation; Secreted, signal peptide; Vesicle; Endoplasmic reticulum; Secreted

Chromosomal Location of Human Ortholog: 11q23-q24

Cellular Component: extracellular space; chylomicron; cell surface; endoplasmic reticulum lumen; endocytic vesicle; early endosome; plasma membrane; extracellular region; cytoplasmic vesicle; nucleus; cytosol; vesicle

Molecular Function: identical protein binding; lipase inhibitor activity; beta-amyloid binding; cholesterol binding; phosphatidylcholine binding; high-density lipoprotein binding; protein binding; enzyme binding; phospholipid transporter activity; cholesterol transporter activity; chemorepellent activity; phospholipid binding; apolipoprotein A-I receptor binding; apolipoprotein receptor binding

Biological Process: phototransduction, visible light; negative chemotaxis; negative regulation of lipase activity; axon regeneration in the peripheral nervous system; sequestering of lipid; negative regulation of interleukin-1 beta secretion; regulation of cholesterol absorption; transforming growth factor beta receptor signaling pathway; positive regulation of stress fiber formation; response to drug; cholesterol metabolic process; platelet activation; organ regeneration; regulation of Cdc42 protein signal transduction; adrenal gland development; positive regulation of hydrolase activity; positive regulation of Rho protein signal transduction; lipoprotein metabolic process; positive regulation of transferase activity; vitamin transport; cholesterol biosynthetic process; negative regulation of cytokine secretion during immune response; cholesterol homeostasis; lipoprotein biosynthetic process; response to estrogen stimulus; peptidyl-methionine modification; phosphatidylcholine biosynthetic process; positive regulation of lipoprotein lipase activity; blood vessel endothelial cell migration; cellular lipid metabolic process; platelet degranulation; phospholipid efflux; retinoid metabolic process; transmembrane transport; response to nutrient; phospholipid homeostasis; integrin-mediated signaling pathway; receptor-mediated endocytosis; positive regulation of fatty acid biosynthetic process; regulation of protein amino acid phosphorylation; cholesterol transport; protein amino acid oxidation; negative regulation of heterotypic cell-cell adhesion; protein stabilization; neurite regeneration; cholesterol efflux; G-protein coupled receptor protein signaling pathway; glucocorticoid metabolic process; reverse cholesterol transport; endothelial cell proliferation; negative regulation of inflammatory response; blood coagulation

Disease: Hypoalphalipoproteinemia, Primary; Amyloidosis, Familial Visceral