Rabbit CBFA2T2 Polyclonal Antibody | anti-CBFA2T2 antibody

CBFA2T2 Antibody

Cat. Num. AAA9611632

• Gene Names: CBFA2T2; EHT; p85; MTGR1; ZMYND3
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: CBFA2T2; Polyclonal Antibody; CBFA2T2 Antibody; AML1 MTG8; cbfa2t2; Core binding factor runt domain alpha subunit 2 translocated to 2; core-binding factor; runt domain; alpha subunit 2; DKFZp313F2116; EHT; ETO homolog on chromosome 20; ETO homologous on chromosome 20; MTG 8 like protein; MTG 8 related protein 1; MTG8 like protein; MTG8 related protein 1; MTG8-like protein; MTG8-related protein 1; MTG8R_HUMAN; MTGR 1; Myeloid translocation gene related protein 1; Myeloid translocation related protein 1; Myeloid translocation-related protein 1; p85; Protein CBFA2T2; ZMYND 3; ZMYND3; anti-CBFA2T2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CBFA2T2 Antibody detects endogenous levels of CBFA2T2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 595

• Applicable Applications for anti-CBFA2T2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human CBFA2T2.
• Tissue Specificity: Ubiquitously expressed in fetal and adult tissues. Highly expressed in adult brain, heart, lung, kidney, lymph node, appendix, thymus, testis, uterus, small intestine, prostate and thymus.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from PC12, using CBFA2T2 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 65 kDa.)

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, using CBFA2T2 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-CBFA2T2 antibody

Description: Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes (PubMed:12559562, PubMed:15203199). Via association with PRDM14 is involved in regulation of embryonic stem cell (ESC) pluripotency (PubMed:27281218). Involved in primordial germ cell (PCG) formation. Stabilizes PRDM14 and OCT4 on chromatin in a homooligomerization-dependent manner (By similarity). Can repress the expression of MMP7 in a ZBTB33-dependent manner (PubMed:23251453). May function as a complex with the chimeric protein RUNX1/AML1-CBFA2T1/MTG8 (AML1-MTG8/ETO fusion protein) which is produced in acute myeloid leukemia with the chromosomal translocation t(8;21). May thus be involved in the repression of AML1-dependent transcription and the induction of G-CSF/CSF3-dependent cell growth. May be a tumor suppressor gene candidate involved in myeloid tumors with the deletion of the 20q11 region. Through heteromerization with CBFA2T3/MTG16 may be involved in regulation of the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes (By similarity). Required for the maintenance of the secretory cell lineage in the small intestine. Can inhibit Notch signaling probably by association with RBPJ and may be involved in GFI1-mediated Paneth cell differentiation (By similarity).

Subunit Structure: Homooligomer. Homotetramerization is mediated by nervy homology region 2 (By similarity). Can interact with RUNX1T1/CBFA2T1 and CBFA2T3/MTG16; heterotetramerization between members of the CBFA2T family is proposed (PubMed:12242670, PubMed:14703694, PubMed:16616331). Forms a heterooligomer with the AML1-MTG8/ETO fusion protein (PubMed:9447981). Interacts with PRDM14 (PubMed:27281218). Interacts with RBPJ, GFI1, TCF4. Interacts with TAL1 and CBFA2T3/MTG16; the heteromer with CBFA2T3/MTG16 may function in repression of TAL1 (By similarity).

Similarity: Nervy homology region 2 (NHR2) mediates homo- and possibly heterotypic oligomerization by forming a four-helix bundle tetrameric structure. Belongs to the CBFA2T family.

NCBI and Uniprot Product Information

• NCBI GI #: 74229008
• NCBI GeneID: 9139
• NCBI Accession #: NP_001028171
• NCBI GenBank Nucleotide #: NP_001028171.1
• UniProt Accession #: O43439
• Molecular Weight: Observed: 80 kDa; Predicted: 68 kDa
• NCBI Official Full Name: protein CBFA2T2 isoform MTGR1c
• NCBI Official Synonym Full Names: CBFA2/RUNX1 partner transcriptional co-repressor 2
• NCBI Official Symbol: CBFA2T2
• NCBI Official Synonym Symbols: EHT; p85; MTGR1; ZMYND3
• NCBI Protein Information: protein CBFA2T2
• UniProt Protein Name: Protein CBFA2T2
• Protein Family: Protein
• UniProt Gene Name: CBFA2T2
• UniProt Synonym Gene Names: EHT; MTGR1
• UniProt Entry Name: MTG8R_HUMAN

NCBI Description

In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described. [provided by RefSeq, Jul 2008]