Monkey Apolipoprotein A1 ELISA Kit | APOA1 elisa kit

Monkey Apolipoprotein A1 ELISA Kit

Cat. Num. AAA739403

• Reactivity: Monkey
• Synonyms: Apolipoprotein A1; Monkey Apolipoprotein A1 ELISA Kit; APOA1 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 50-1000ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This APOA1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey APOA1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOA1 elisa kit

Principle of the Assay: APOA1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for APOA1. Standards or samples are then added to the microtiter plate wells and APOA1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of APOA1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for APOA1 are added to each well to "sandwich" the APOA1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain APOA1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APOA1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for APOA1 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 4557321
• NCBI GeneID: 335
• NCBI Accession #: NP_000030.1
• NCBI GenBank Nucleotide #: NM_000039.1
• Molecular Weight: 30,778 Da
• NCBI Official Full Name: apolipoprotein A-I preproprotein
• NCBI Official Synonym Full Names: apolipoprotein A-I
• NCBI Official Symbol: APOA1
• NCBI Protein Information: apolipoprotein A-I; apo-AI
• UniProt Protein Name: Apolipoprotein A-I
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA1
• UniProt Synonym Gene Names: Apo-AI; ApoA-I; ProapoA-I
• UniProt Entry Name: APOA1_HUMAN

NCBI Description

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq, Jul 2008]

Uniprot Description

APOA1: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Secreted, signal peptide; Cell development/differentiation; Motility/polarity/chemotaxis; Vesicle; Endoplasmic reticulum; Lipid-binding; Secreted

Chromosomal Location of Human Ortholog: 11q23-q24

Cellular Component: extracellular space; chylomicron; cell surface; endocytic vesicle; endoplasmic reticulum lumen; early endosome; plasma membrane; extracellular region; cytoplasmic vesicle; nucleus; cytosol; vesicle

Molecular Function: identical protein binding; lipase inhibitor activity; beta-amyloid binding; cholesterol binding; phosphatidylcholine binding; high-density lipoprotein binding; protein binding; enzyme binding; phospholipid transporter activity; chemorepellent activity; cholesterol transporter activity; phospholipid binding; apolipoprotein A-I receptor binding; apolipoprotein receptor binding

Biological Process: phototransduction, visible light; blood vessel endothelial cell migration; negative chemotaxis; cellular lipid metabolic process; negative regulation of lipase activity; axon regeneration in the peripheral nervous system; negative regulation of interleukin-1 beta secretion; sequestering of lipid; platelet degranulation; phospholipid efflux; regulation of cholesterol absorption; transforming growth factor beta receptor signaling pathway; positive regulation of stress fiber formation; retinoid metabolic process; transmembrane transport; response to nutrient; phospholipid homeostasis; response to drug; integrin-mediated signaling pathway; positive regulation of fatty acid biosynthetic process; receptor-mediated endocytosis; cholesterol metabolic process; platelet activation; regulation of protein amino acid phosphorylation; organ regeneration; regulation of Cdc42 protein signal transduction; cholesterol transport; negative regulation of heterotypic cell-cell adhesion; protein stabilization; protein amino acid oxidation; adrenal gland development; positive regulation of hydrolase activity; positive regulation of Rho protein signal transduction; neurite regeneration; cholesterol efflux; lipoprotein metabolic process; positive regulation of transferase activity; vitamin transport; cholesterol biosynthetic process; negative regulation of cytokine secretion during immune response; glucocorticoid metabolic process; G-protein coupled receptor protein signaling pathway; cholesterol homeostasis; reverse cholesterol transport; response to estrogen stimulus; lipoprotein biosynthetic process; peptidyl-methionine modification; negative regulation of inflammatory response; endothelial cell proliferation; phosphatidylcholine biosynthetic process; positive regulation of lipoprotein lipase activity; blood coagulation

Disease: Hypoalphalipoproteinemia, Primary; Amyloidosis, Familial Visceral