Typical Testing Data/Standard Curve (for reference only)
Mouse Apelin Receptor (APLNR) ELISA Kit | APLNR elisa kit
Mouse Apelin Receptor (APLNR) ELISA Kit
Gene Names
Aplnr; APJ; Agtrl1; msr/apj
Reactivity
Mouse
Synonyms
Apelin Receptor (APLNR); Mouse Apelin Receptor (APLNR) ELISA Kit; AGTRL1; HG11; APJR; APJ; APJ Receptor; Angiotensin II Receptor-Like 1; Angiotensin receptor-like 1; APLNR elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of APLNR. No significant cross-reactivity or interference between APLNR and analogues was observed.
Sequence Length
377
Samples
Mouse tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
15.6-1000pg/mL
Sensitivity
< 5.9pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level APLNR were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level APLNR were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for APLNR elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of APLNR in mouse tissue homogenates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to APLNR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to APLNR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain APLNR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of APLNR in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to APLNR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to APLNR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain APLNR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of APLNR in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,266 Da
NCBI Official Full Name
apelin receptor
NCBI Official Synonym Full Names
apelin receptor
NCBI Official Symbol
Aplnr
NCBI Official Synonym Symbols
APJ; Agtrl1; msr/apj
NCBI Protein Information
apelin receptor
UniProt Protein Name
Apelin receptor
Protein Family
UniProt Gene Name
Aplnr
UniProt Synonym Gene Names
Agtrl1; Apj
Uniprot Description
Receptor for apelin receptor early endogenous ligand (APELA) and apelin (APLN) hormones coupled to G proteins that inhibit adenylate cyclase activity. Plays a key role in early development such as gastrulation and heart morphogenesis by acting as a receptor for APELA hormone. Plays also a role in various processes in adults such as regulation of blood vessel formation, blood pressure, heart contractility, and heart failure by acting as a receptor for APLN hormone.
Research Articles on APLNR
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Product Notes
The Mouse APLNR aplnr (Catalog #AAA456961) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA456961 ELISA Kit recognizes Mouse APLNR. It is sometimes possible for the material contained within the vial of "Apelin Receptor (APLNR), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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