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Typical Testing Data/Standard Curve (for reference only)

Human apelin receptor ELISA Kit | APLNR elisa kit

Human Apelin receptor, APLNR ELISA Kit

Gene Names
APLNR; APJ; APJR; HG11; AGTRL1
Reactivity
Human
Synonyms
apelin receptor; Human Apelin receptor; APLNR ELISA Kit; Human Apelin receptor (APLNR) ELISA kit; AGTRL1; APJ; APJR; FLJ90771; FLJ96609; HG11; MGC45246; APJ (apelin) receptor; APJ receptor; G protein-coupled receptor APJ; HG11 orphan receptor; angiotensin II receptor-like 1; angiotensin receptor-like; APLNR elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human APLNR. No significant cross-reactivity or interference between human APLNR and analogues was observed.
Sequence Length
380
Samples
Serum, plasma, tissue homogenates, cell lysates.
Assay Type
Sandwich
Detection Range
23.5 pg/ml -1500 pg/ml.
Sensitivity
5.8 pg/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for APLNR elisa kit
Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for APLNR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any APLNR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APLNR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APLNR bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
187
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42,660 Da
NCBI Official Full Name
apelin receptor
NCBI Official Synonym Full Names
apelin receptor
NCBI Official Symbol
APLNR
NCBI Official Synonym Symbols
APJ; APJR; HG11; AGTRL1
NCBI Protein Information
apelin receptor; APJ receptor; HG11 orphan receptor; APJ (apelin) receptor; angiotensin receptor-like 1; G protein-coupled receptor APJ; G-protein coupled receptor APJ; angiotensin II receptor-like 1; G-protein coupled receptor HG11
UniProt Protein Name
Apelin receptor
Protein Family
UniProt Gene Name
APLNR
UniProt Synonym Gene Names
AGTRL1; APJ
UniProt Entry Name
APJ_HUMAN

NCBI Description

This gene encodes a member of the G protein-coupled receptor gene family. The encoded protein is related to the angiotensin receptor, but is actually an apelin receptor that inhibits adenylate cyclase activity and plays a counter-regulatory role against the pressure action of angiotensin II by exerting hypertensive effect. It functions in the cardiovascular and central nervous systems, in glucose metabolism, in embryonic and tumor angiogenesis and as a human immunodeficiency virus (HIV-1) coreceptor. Two transcript variants resulting from alternative splicing have been identified. [provided by RefSeq, Jul 2009]

Uniprot Description

AGTRL1: Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; regulation of body fluid levels; heart development; gastrulation

Research Articles on APLNR

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Product Notes

The Human APLNR aplnr (Catalog #AAA928514) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA928514 ELISA Kit recognizes Human APLNR. It is sometimes possible for the material contained within the vial of "apelin receptor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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