Western Blot (WB)
(All lanes : Anti-NOP2 Antibody (N-Term) at 1:2000 dilutionLane 1: K562 whole cell lysateLane 2: U-2OS whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 89 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit NOP2 Polyclonal Antibody | anti-NOP2 antibody
NOP2 Antibody (N-Term)
Gene Names
NOP2; NOL1; p120; NSUN1; NOP120
Reactivity
HumanPredicted: Mouse
Applications
Western Blot, ELISA
Synonyms
NOP2; Polyclonal Antibody; NOP2 Antibody (N-Term); Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase; 2.1.1.-; Nucleolar protein 1; Nucleolar protein 2 homolog; Proliferating-cell nucleolar antigen p120; Proliferation-associated nucleolar protein p120; NOL1; NSUN1; Purified Rabbit Polyclonal Antibody (Pab); anti-NOP2 antibody
Host
Rabbit
Reactivity
Human
Predicted: Mouse
Predicted: Mouse
Clonality
Polyclonal
Isotype
Ig
Specificity
This NOP2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 9-43 amino acids from human NOP2.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
0.76 (varies by lot)
Sequence Length
812
Applicable Applications for anti-NOP2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:2000
Cellular Location
Nucleus, nucleolus
Antigen Type
Synthesized Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(All lanes : Anti-NOP2 Antibody (N-Term) at 1:2000 dilutionLane 1: K562 whole cell lysateLane 2: U-2OS whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 89 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-NOP2 Antibody (N-Term) at 1:2000 dilutionLane 1: K562 whole cell lysateLane 2: U-2OS whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 89 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-NOP2 antibody
S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C(5) position of cytosine 4447 in 28S rRNA (Probable). May play a role in the regulation of the cell cycle and the increased nucleolar activity that is associated with the cell proliferation.
Product Categories/Family for anti-NOP2 antibody
References
Fonagy A.,et al.Cancer Commun. 1:243-251(1989). Larson R.G.,et al.Cancer Commun. 2:63-71(1990). Valdez B.C.,et al.Cancer Res. 52:5681-5686(1992). Ota T.,et al.Nat. Genet. 36:40-45(2004). Scherer S.E.,et al.Nature 440:346-351(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
89302 Da
NCBI Official Full Name
Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase
NCBI Official Synonym Full Names
NOP2 nucleolar protein
NCBI Official Symbol
NOP2
NCBI Official Synonym Symbols
NOL1; p120; NSUN1; NOP120
NCBI Protein Information
probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase
UniProt Protein Name
Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase
Protein Family
UniProt Gene Name
NOP2
UniProt Synonym Gene Names
NOL1; NSUN1
UniProt Entry Name
NOP2_HUMAN
Uniprot Description
NOP2: May play a role in the regulation of the cell cycle and the increased nucleolar activity that is associated with the cell proliferation. May act as ribosomal RNA methyltransferase. Belongs to the methyltransferase superfamily. RsmB/NOP family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.1.1.-; Cell cycle regulation; Nucleolus; Methyltransferase; RNA-binding
Chromosomal Location of Human Ortholog: 12p13
Cellular Component: nucleolus
Molecular Function: protein binding
Biological Process: positive regulation of cell proliferation
Research Articles on NOP2
Similar Products
Product Notes
The NOP2 nop2 (Catalog #AAA9217362) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NOP2 Antibody (N-Term) reacts with Human Predicted: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NOP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:2000. Researchers should empirically determine the suitability of the NOP2 nop2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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