Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Alpha-Melanocyte Stimulating Hormone (A-MSH) Standard Curve.)
Mouse Alpha-Melanocyte Stimulating Hormone (A-MSH) ELISA Kit | A-MSH elisa kit
Mouse Alpha-Melanocyte Stimulating Hormone (A-MSH) ELISA Kit
Gene Names
POMC; alpha-MSH
Reactivity
Mouse
Synonyms
Alpha-Melanocyte Stimulating Hormone (A-MSH); Mouse Alpha-Melanocyte Stimulating Hormone (A-MSH) ELISA Kit; A-MSH; alpha-MSH; Intermedins; Alpha-Melanotropin; Alpha-Melanocortin; Alpha-Intermedin; A-MSH elisa kit
Reactivity
Mouse
Specificity
Mouse Alpha-Melanocyte Stimulating Hormone (A-MSH) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse A-MSH. No significant cross-reactivity or interference between Mouse A-MSH and analogues was observed.
Sequence Length
49
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Alpha-Melanocyte Stimulating Hormone (A-MSH) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Alpha-Melanocyte Stimulating Hormone (A-MSH) Standard Curve.)
Related Product Information for A-MSH elisa kit
Description: This Mouse Alpha-Melanocyte Stimulating Hormone (A-MSH) ELISA Kit employs a two-site sandwich ELISA to quantitate A-MSH in samples. An antibody specific for A-MSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any A-MSH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for A-MSH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of A-MSH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Melanocyte-stimulating hormone belongs to a group called the melanocortins. This group includes ACTH, alpha-MSH, beta-MSH and gamma-MSH; these peptides are all cleavage products of a large precursor peptide called pro-opiomelanocortin (POMC). Alpha-MSH is the most important melanocortin for pigmentation.Alpha-MSH could be a potent biological marker for the identification of CFS. Despite extensive research, no reliable biological marker for chronic fatigue syndrome (CFS) has yet been identified. However, hyperactivation of melanotrophs in the pituitary gland and increased levels of plasma alpha-melanocyte-stimulating hormone (alpha-MSH) have recently been detected in an animal model of chronic stress. Because CFS is considered to be caused partly by chronic stress events, increased alpha-MSH plasma levels may also occur in CFS patients.
Background: Melanocyte-stimulating hormone belongs to a group called the melanocortins. This group includes ACTH, alpha-MSH, beta-MSH and gamma-MSH; these peptides are all cleavage products of a large precursor peptide called pro-opiomelanocortin (POMC). Alpha-MSH is the most important melanocortin for pigmentation.Alpha-MSH could be a potent biological marker for the identification of CFS. Despite extensive research, no reliable biological marker for chronic fatigue syndrome (CFS) has yet been identified. However, hyperactivation of melanotrophs in the pituitary gland and increased levels of plasma alpha-melanocyte-stimulating hormone (alpha-MSH) have recently been detected in an animal model of chronic stress. Because CFS is considered to be caused partly by chronic stress events, increased alpha-MSH plasma levels may also occur in CFS patients.
NCBI and Uniprot Product Information
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Product Notes
The Mouse A-MSH (Catalog #AAA9718113) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718113 ELISA Kit recognizes Mouse A-MSH. It is sometimes possible for the material contained within the vial of "Alpha-Melanocyte Stimulating Hormone (A-MSH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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