Rat Adrenocorticotropic Hormone (ACTH) ELISA Kit | ACTH elisa kit

Rat Adrenocorticotropic Hormone (ACTH) ELISA Kit

Cat. Num. AAA9711958

• Gene Names: Pomc; BE; Npp; ACTH; Clip; Pomc1; Pomc-1; Beta-LPH; alphaMSH; beta-MSH; Gamma-LPH; alpha-MSH; gamma-MSH
• Reactivity: Rat
• Synonyms: Adrenocorticotropic Hormone (ACTH); Rat Adrenocorticotropic Hormone (ACTH) ELISA Kit; ACTH elisa kit

• Reactivity: Rat
• Specificity: Rat Adrenocorticotropic hormone (ACTH) ELISA Kit has high sensitivity and excellent specificity for detection of Rat ACTH. No significant cross-reactivity or interference between Rat ACTH and analogues was observed.
• Sequence Length: 39

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Rat Adrenocorticotropic hormone (ACTH) Standard Curve.)

Related Product Information for ACTH elisa kit

Description: This Rat Adrenocorticotropic hormone (ACTH) ELISA Kit employs a two-site sandwich ELISA to quantitate ACTH in samples. An antibody specific for ACTH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACTH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACTH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: ACTH (Adrenocorticotropic hormone) or corticotropin is a 39-amino acid peptide hormone (MW=4500) secreted by the pituitary to regulate the production of steroid hormones by the adrenal cortex. ACTH secretion from the anterior pituitary is controlled by both a classical negative feedback control mechanism and CNS-stress mediated control system. Various types of stress or pain perceived in higher levels of the brain modulate secretion of the hypothalamic neurosecretory hormone, corticotropin releasing hormone (CRH), a 41-amino acid peptide. CRH stimulates pituitary ACTH secretion. The second peptide that modulates ACTH secretion is vasopressin (AVP). AVP secretion is also stimulated by stress and acts synergistically with CRH to increase ACTH secretion in the pituitary portal circulation. ACTH increases the synthesis and release of all adrenal sterioids, aldosterone, cortisol and adrenal androgens.

NCBI and Uniprot Product Information

• NCBI GI #: 191659
• NCBI GeneID: 18976
• NCBI Official Full Name: adrenocorticotropic hormone, partial
• NCBI Official Synonym Full Names: pro-opiomelanocortin-alpha
• NCBI Official Symbol: Pomc
• NCBI Official Synonym Symbols: BE; Npp; ACTH; Clip; Pomc1; Pomc-1; Beta-LPH; alphaMSH; beta-MSH; Gamma-LPH; alpha-MSH; gamma-MSH
• NCBI Protein Information: pro-opiomelanocortin

NCBI Description

This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing. Processing yields several biologically active peptides, which are involved in diverse cellular functions, such as energy homeostasis, steroidogenesis, and increased melanin production in melanocytes. In mouse deficiency of this gene is associated with obesity, defects in adrenal development, and altered pigmentation. A pseudogene of this gene is located on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]