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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Anti-Follicle-Stimulating Hormone Antibody (Anti-FSH-Ab) Standard Curve.)

Human Follicle-Stimulating Hormone (Anti-FSH-Ab) ELISA Kit | FSH-Ab elisa kit

Human Anti-Follicle-Stimulating Hormone Antibody (Anti-FSH-Ab) ELISA Kit

Reactivity
Human
Synonyms
Follicle-Stimulating Hormone (Anti-FSH-Ab); Human Anti-Follicle-Stimulating Hormone Antibody (Anti-FSH-Ab) ELISA Kit; FSH-Ab elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Human Anti-Follicle-Stimulating Hormone Antibody (Anti-FSH-Ab) ELISA Kit has high sensitivity and excellent specificity for detection of Human Anti-FSH-Ab. No significant cross-reactivity or interference between Human Anti-FSH-Ab and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Anti-Follicle-Stimulating Hormone Antibody (Anti-FSH-Ab) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Anti-Follicle-Stimulating Hormone Antibody (Anti-FSH-Ab) Standard Curve.)
Related Product Information for FSH-Ab elisa kit
Description: This Human Anti-Follicle-Stimulating Hormone Antibody (Anti-FSH-Ab) ELISA Kit employs a two-site sandwich ELISA to quantitate Anti-FSH-Ab in samples. An antibody specific for Anti-FSH-Ab has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Anti-FSH-Ab present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Anti-FSH-Ab is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-FSH-Ab bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Follicle stimulating hormone (FSH) is a hormone synthesised and secreted by gonadotropes in the anterior pituitary gland. In the ovary FSH stimulates the growth of immature Graafian follicles to maturation. FSH is a glycoprotein secreted by the basophil cells of the anterior pituitary. Gonadotropin-releasing hormone (GnRH), produced in the hypothalamus, controls the release of FSH from the anterior pituitary. Like other glycoproteins, such as LH, TSH, and HCG, FSH consists of subunits designated as alpha and beta. Hormones of this type have alpha subunits that are very similar in structure; therefore the biological and immunological properties of each hormone are dependent on the unique beta subunit. In the female, FSH stimulates the growth and maturation of ovarian follicles by acting directly on the receptors located on the granulosa cells; follicular steroidogenesis is promoted and LH production is stimulated.

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Product Notes

The Human FSH-Ab (Catalog #AAA9716118) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716118 ELISA Kit recognizes Human FSH-Ab. It is sometimes possible for the material contained within the vial of "Follicle-Stimulating Hormone (Anti-FSH-Ab), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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