Mouse Alpha-2-Macroglobulin (A2M) ELISA Kit | A2M elisa kit

Mouse Alpha-2-Macroglobulin (A2M) ELISA Kit

Cat. Num. AAA9717130

• Reactivity: Mouse
• Synonyms: Alpha-2-Macroglobulin (A2M); Mouse Alpha-2-Macroglobulin (A2M) ELISA Kit; A2M; CPAMD5; DKFZp779B086; FWP007; S863-7; A2M elisa kit

• Reactivity: Mouse
• Specificity: Mouse Alpha-2-macroglobulin (A2M) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse A2M. No significant cross-reactivity or interference between Mouse A2M and analogues was observed.
• Sequence Length: 1495

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Alpha-2-macroglobulin (A2M) Standard Curve.)

Related Product Information for A2M elisa kit

Description: This Mouse Alpha-2-macroglobulin (A2M) ELISA Kit employs a two-site sandwich ELISA to quantitate A2M in samples. An antibody specific for A2M has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any A2M present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for A2M is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of A2M bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Alpha2-macroglobulin is a major serum protein with diverse functions, including inhibition of protease activity and binding of growth factors, cytokines, and disease factors. It is also a panproteinase inhibitor that is found immunohistochemically in neuritic plaques. Alpha-2-macroglobulin is able to inactivate an enormous variety of proteinases (including serine-, cysteine-, aspartic- and metalloproteinases). Alpha-2-macroglobulin has in its structure a 35 aminoacid "bait" region. Proteinases binding and cleaving the bait region become bound to alpha2M. The proteinase-alpha2M complex is recognised by macrophage receptors and cleared from the system

NCBI and Uniprot Product Information

• NCBI GI #: 338817897
• UniProt Accession #: Q61838
• NCBI Official Full Name: Pregnancy zone protein
• UniProt Protein Name: Pregnancy zone protein
• Protein Family: Alpha-2-macroglobulin
• UniProt Gene Name: Pzp
• UniProt Synonym Gene Names: A2m; Alpha-2-M

Uniprot Description

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.