Typical Testing Data/Standard Curve (for reference only)
Mouse Aldolase A, Fructose Bisphosphate (ALDOA) ELISA Kit | ALDOA elisa kit
Mouse Aldolase A, Fructose Bisphosphate (ALDOA) ELISA Kit
Gene Names
Aldoa; Aldo1; Aldo-1
Reactivity
Mouse
Synonyms
Aldolase A; Fructose Bisphosphate (ALDOA); Mouse Aldolase A; Fructose Bisphosphate (ALDOA) ELISA Kit; FBA; ALDO-A; Fructose 1; 6 Bisphosphate Aldolase; Lung cancer antigen NY-LU-1; Muscle-type aldolase; ALDOA elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of ALDOA. No significant cross-reactivity or interference between ALDOA and analogues was observed.
Sequence Length
364
Samples
Serum, plasma, tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
78.125-5000pg/mL
Sensitivity
<34.5pg/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ALDOA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ALDOA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ALDOA elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ALDOA in mouse serum, plasma, tissue homogenates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ALDOA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ALDOA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ALDOA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ALDOA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ALDOA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ALDOA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ALDOA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ALDOA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39,356 Da
NCBI Official Full Name
fructose-bisphosphate aldolase A isoform 2
NCBI Official Synonym Full Names
aldolase A, fructose-bisphosphate
NCBI Official Symbol
Aldoa
NCBI Official Synonym Symbols
Aldo1; Aldo-1
NCBI Protein Information
fructose-bisphosphate aldolase A
UniProt Protein Name
Fructose-bisphosphate aldolase A
Protein Family
UniProt Gene Name
Aldoa
UniProt Synonym Gene Names
Aldo1
Uniprot Description
Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein ().
Research Articles on ALDOA
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Product Notes
The Mouse ALDOA aldoa (Catalog #AAA456946) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA456946 ELISA Kit recognizes Mouse ALDOA. It is sometimes possible for the material contained within the vial of "Aldolase A, Fructose Bisphosphate (ALDOA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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