Rabbit anti-Human SESN1 Polyclonal Antibody | anti-SESN1 antibody

SESN1 Antibody

Cat. Num. AAA7052389

• Gene Names: SESN1; PA26; SEST1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >95%, Protein G purified
• Synonyms: SESN1; Polyclonal Antibody; SESN1 Antibody; Sestrin-1; p53-regulated protein PA26; anti-SESN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 492

• Applicable Applications for anti-SESN1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:5000; IHC: 1:20-1:200
• Immunogen: Recombinant Human Sestrin-1 protein (224-337AA)
• Conjugation: Non-conjugated
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: K562 whole cell lysate,Hela whole cell lysate,NIH/3T3 whole cell lysateAll lanes: SESN1 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 57,64,50 KDaObserved band size: 57 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human breast cancer using MBS7052389 at dilution of 1:100)

Related Product Information for anti-SESN1 antibody

Functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway (PubMed:25263562, PubMed:26449471). This stress-inducible metabolic regulator may also play a role in protection against oxidative and genotoxic stresses (By similarity). May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1 (PubMed:23274085). May have an alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (By similarity). Was originally reported to contribute to oxidative stress resistance by reducing PRDX1 (PubMed:15105503). However, this could not be confirmed (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 315709500
• NCBI GeneID: 27244
• NCBI Accession #: NP_001186862.1
• NCBI GenBank Nucleotide #: NM_001199933.1
• UniProt Accession #: Q9Y6P5; Q2M2B7; Q5T316; Q9NV00; Q9UPD5; Q9Y6P6
• Molecular Weight: 49,624 Da
• NCBI Official Full Name: sestrin-1 isoform 2
• NCBI Official Synonym Full Names: sestrin 1
• NCBI Official Symbol: SESN1
• NCBI Official Synonym Symbols: PA26; SEST1
• NCBI Protein Information: sestrin-1
• UniProt Protein Name: Sestrin-1
• Protein Family: Sestrin
• UniProt Gene Name: SESN1

NCBI Description

This gene encodes a member of the sestrin family. Sestrins are induced by the p53 tumor suppressor protein and play a role in the cellular response to DNA damage and oxidative stress. The encoded protein mediates p53 inhibition of cell growth by activating AMP-activated protein kinase, which results in the inhibition of the mammalian target of rapamycin protein. The encoded protein also plays a critical role in antioxidant defense by regenerating overoxidized peroxiredoxins, and the expression of this gene is a potential marker for exposure to radiation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

SESN1: Involved in the reduction of peroxiredoxins. May also be regulator of cellular growth. Belongs to the sestrin family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: protein binding