Human Neuregulin 3 ELISA Kit | NRG-3 elisa kit

Human Neuregulin 3 ELISA Kit

Cat. Num. AAA764191

• Gene Names: NRG3; HRG3; pro-NRG3
• Reactivity: Human
• Synonyms: Neuregulin 3; Human Neuregulin 3 ELISA Kit; NRG-3; NRG-3 elisa kit

• Reactivity: Human
• Sequence Length: 499

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for NRG-3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human NRG-3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human NRG-3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human NRG-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human NRG-3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 110611260
• NCBI GeneID: 10718
• Molecular Weight: 75,031 Da
• NCBI Official Full Name: neuregulin 3
• NCBI Official Synonym Full Names: neuregulin 3
• NCBI Official Symbol: NRG3
• NCBI Official Synonym Symbols: HRG3; pro-NRG3
• NCBI Protein Information: pro-neuregulin-3, membrane-bound isoform
• UniProt Protein Name: Pro-neuregulin-3, membrane-bound isoform
• UniProt Gene Name: NRG3
• UniProt Synonym Gene Names: Pro-NRG3; NRG-3
• UniProt Entry Name: NRG3_HUMAN

NCBI Description

This gene is a member of the neuregulin gene family. This gene family encodes ligands for the transmembrane tyrosine kinase receptors ERBB3 and ERBB4 - members of the epidermal growth factor receptor family. Ligand binding activates intracellular signaling cascades and the induction of cellular responses including proliferation, migration, differentiation, and survival or apoptosis. This gene encodes neuregulin 3 (NRG3). NRG3 has been shown to activate the tyrosine phosphorylation of its cognate receptor, ERBB4, and is thought to influence neuroblast proliferation, migration and differentiation by signalling through ERBB4. NRG3 also promotes mammary differentiation during embryogenesis. Linkage studies have implicated this gene as a susceptibility locus for schizophrenia and schizoaffective disorder. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but their biological validity has not been verified.[provided by RefSeq, Sep 2009]

Uniprot Description

NRG3: Direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes. Belongs to the neuregulin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligand, receptor tyrosine kinase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q23.1

Cellular Component: extracellular region; extracellular space; integral to plasma membrane; intracellular

Molecular Function: chemorepellent activity; growth factor activity; receptor binding; receptor tyrosine kinase binding; transmembrane receptor protein tyrosine kinase activator activity

Biological Process: chemorepulsion involved in interneuron migration from the subpallium to the cortex; organ development; pattern specification process; regulation of cell growth; transmembrane receptor protein tyrosine kinase activation (dimerization)