Rabbit anti-Human, Mouse ATP5G1 Polyclonal Antibody | anti-ATP5G1 antibody

ATP5G1, ID (ATP5G1, ATP synthase lipid-binding protein, mitochondrial, ATP synthase proteolipid P1, ATPase protein 9, ATPase subunit c) (PE)

Cat. Num. AAA6276545

• Gene Names: ATP5G1; ATP5A; ATP5G
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ATP5G1; Polyclonal Antibody; ID (ATP5G1; ATP synthase lipid-binding protein; mitochondrial; ATP synthase proteolipid P1; ATPase protein 9; ATPase subunit c) (PE); ATPase subunit c; anti-ATP5G1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ATP5G1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ATP5G1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-56 amino acids from the Central region of human ATP5G1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ATP5G1 Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the ATP5G1 antibody detected the ATP5G1 protein (arrow).)

Product Categories/Family for anti-ATP5G1 antibody

Antibodies; Mitochondrial Proteins

References

Vives-Bauza, C., et al. Mol. Biol. Cell 21(1):131-139(2010). Wang, H.L., et al. Cytogenet. Genome Res. 109 (4), 533 (2005): Cross, R.L. Nature 427(6973):407-408(2004). Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000). Wang, H., et al. Nature 396(6708):279-282(1998).

NCBI and Uniprot Product Information

• NCBI GI #: 50659069
• NCBI GeneID: 516
• NCBI Accession #: NP_001002027.1; NP_005166.1
• NCBI GenBank Nucleotide #: NM_001002027.1
• UniProt Accession #: P05496
• Molecular Weight: 14,277 Da
• NCBI Official Full Name: ATP synthase F(0) complex subunit C1, mitochondrial
• NCBI Official Synonym Full Names: ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C1 (subunit 9)
• NCBI Official Symbol: ATP5G1
• NCBI Official Synonym Symbols: ATP5A; ATP5G
• NCBI Protein Information: ATP synthase F(0) complex subunit C1, mitochondrial; ATP synthase F(0) complex subunit C1, mitochondrial; ATPase protein 9; ATPase subunit 9; ATPase subunit C; ATP synthase proteolipid P1; mitochondrial ATP synthase, subunit 9, isoform 1; mitochondrial AT
• UniProt Protein Name: ATP synthase F(0) complex subunit C1, mitochondrial
• UniProt Gene Name: ATP5G1
• UniProt Entry Name: AT5G1_HUMAN

NCBI Description

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene is one of three genes that encode subunit c of the proton channel. Each of the three genes have distinct mitochondrial import sequences but encode the identical mature protein. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]