Mouse Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) DIY ELISA Kit | TRAIL diy elisa kit

Mouse Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) ELISA Kit DIY Materials

Cat. Num. AAA2088437

• Gene Names: Tnfsf10; TL2; Ly81; Trail; APO-2L; Tnlg6a; AI448571; A330042I21Rik
• Reactivity: Mouse
• Synonyms: Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL); Mouse Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) ELISA Kit DIY Materials; CD253; TNFSF10; APO2L; Apo2-L; TL2; TRAIL; Tumor Necrosis Factor Ligand Superfamily Member 10; Apo-2 Ligand; TRAIL diy elisa kit

• Reactivity: Mouse
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL). No significant cross-reactivity or interference between Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) and analogues was observed.
• Sequence Length: 291

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
• Detection Range: 15.6-1000pg/mL
• Sensitivity: 6.2pg/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for TRAIL diy elisa kit

Apoptosis; Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 6678431
• NCBI GeneID: 22035
• NCBI Accession #: NP_033451.1
• NCBI GenBank Nucleotide #: NM_009425.2
• UniProt Accession #: P50592
• Molecular Weight: 33,477 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 10
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 10
• NCBI Official Symbol: Tnfsf10
• NCBI Official Synonym Symbols: TL2; Ly81; Trail; APO-2L; Tnlg6a; AI448571; A330042I21Rik
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 10
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 10
• UniProt Gene Name: Tnfsf10
• UniProt Synonym Gene Names: Trail; Protein TRAIL

Uniprot Description

TRAIL: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. Homotrimer. Widespread; most predominant in spleen, lung and prostate. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3|3 A3

Molecular Function: tumor necrosis factor receptor superfamily binding

Biological Process: positive regulation of apoptosis; positive regulation of caspase activity