Mouse Enolase, Non Neuronal (NNE) DIY ELISA Kit | NNE diy elisa kit

Mouse Enolase, Non Neuronal (NNE) ELISA Kit DIY Materials

Cat. Num. AAA2089098

• Gene Names: Eno1b; Eno1; Gm5506; EG433182
• Reactivity: Mouse
• Synonyms: Enolase; Non Neuronal (NNE); Mouse Enolase; Non Neuronal (NNE) ELISA Kit DIY Materials; ENO1; ENO1L1; MPB1; PPH; Alpha Enolase; Enolase 1; Phosphopyruvate hydratase; Plasminogen-binding protein; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding; NNE diy elisa kit

• Reactivity: Mouse
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Enolase, Non Neuronal (NNE). No significant cross-reactivity or interference between Enolase, Non Neuronal (NNE) and analogues was observed.
• Sequence Length: 434

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma and other biological fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.114ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for NNE diy elisa kit

Enzyme & Kinase; Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 70794816
• NCBI GeneID: 433182
• NCBI Accession #: NP_001020559.1
• NCBI GenBank Nucleotide #: NM_001025388.1
• UniProt Accession #: P17182; Q99KT7; Q9DCY7
• Molecular Weight: 47,141 Da
• NCBI Official Full Name: enolase 1B, retrotransposed
• NCBI Official Synonym Full Names: enolase 1B, retrotransposed
• NCBI Official Symbol: Eno1b
• NCBI Official Synonym Symbols: Eno1; Gm5506; EG433182
• NCBI Protein Information: enolase 1B, retrotransposed
• UniProt Protein Name: Alpha-enolase
• UniProt Gene Name: Eno1
• UniProt Synonym Gene Names: Eno-1; NNE

NCBI Description

This gene may represent an evolving pseudogene of the alpha-enolase (enolase 1, alpha non-neuron) gene, which has multiple pseudogenes. This gene has an intact open reading frame as well as strong transcriptional support. The length of encoded protein is conserved, compared to the original enolase 1 protein. The exact function of this gene is unknown. [provided by RefSeq, Jul 2008]

Uniprot Description

ENO1: an abundant cytoplasmic enzyme with 2-phospho-D-glycerate hydro-lyase activity. A target of excess protein nitration in Alzheimer disease (AD) brain. Tau-crystallin, a structural lens protein, is produced from an alternative translation start in the enolase gene.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.2.1.11; Lyase; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 4 E2|4 81.24 cM

Cellular Component: cell-cell adherens junction; cytoplasm; extracellular space; extrinsic to plasma membrane; membrane; myelin sheath; neuron projection; nucleus

Molecular Function: GTPase binding; heat shock protein binding; phosphopyruvate hydratase activity; protein heterodimerization activity; protein homodimerization activity; RNA binding

Biological Process: in utero embryonic development; positive regulation of binding