Mouse Enolase, Non Neuronal (NNE) ELISA Kit | NNE elisa kit

Mouse Enolase, Non Neuronal (NNE) ELISA Kit

Cat. Num. AAA2097959

• Gene Names: Eno1b; Eno1; Gm5506; EG433182
• Reactivity: Mouse
• Synonyms: Enolase; Non Neuronal (NNE); Mouse Enolase; Non Neuronal (NNE) ELISA Kit; ENO1; ENO1L1; MPB1; PPH; Alpha Enolase; Enolase 1; Phosphopyruvate hydratase; Plasminogen-binding protein; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding; NNE elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Enolase, Non Neuronal (NNE).; No significant cross-reactivity or interference between Enolase, Non Neuronal (NNE) and analogues was observed.
• Sequence Length: 434

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 78-5,000pg/mL
• Sensitivity: < 31pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Enolase, Non Neuronal (NNE) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Enolase, Non Neuronal (NNE) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NNE elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Enolase, Non Neuronal (NNE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Enolase, Non Neuronal (NNE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Enolase, Non Neuronal (NNE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Enolase, Non Neuronal (NNE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for NNE elisa kit

Enzyme & Kinase; Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 70794816
• NCBI GeneID: 433182
• NCBI Accession #: NP_001020559.1
• NCBI GenBank Nucleotide #: NM_001025388.1
• UniProt Accession #: P17182; Q99KT7; Q9DCY7
• Molecular Weight: 47,141 Da
• NCBI Official Full Name: enolase 1B, retrotransposed
• NCBI Official Synonym Full Names: enolase 1B, retrotransposed
• NCBI Official Symbol: Eno1b
• NCBI Official Synonym Symbols: Eno1; Gm5506; EG433182
• NCBI Protein Information: enolase 1B, retrotransposed
• UniProt Protein Name: Alpha-enolase
• UniProt Gene Name: Eno1
• UniProt Synonym Gene Names: Eno-1; NNE

NCBI Description

This gene may represent an evolving pseudogene of the alpha-enolase (enolase 1, alpha non-neuron) gene, which has multiple pseudogenes. This gene has an intact open reading frame as well as strong transcriptional support. The length of encoded protein is conserved, compared to the original enolase 1 protein. The exact function of this gene is unknown. [provided by RefSeq, Jul 2008]

Uniprot Description

Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses (). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production ().