Mouse FMS Like Tyrosine Kinase 3 Ligand (Flt3L) CLIA Kit | Flt3L clia kit

Mouse FMS Like Tyrosine Kinase 3 Ligand (Flt3L) CLIA Kit

Cat. Num. AAA2708532

• Gene Names: Flt3l; Ly72L; Flt3lg
• Reactivity: Mouse
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: FMS Like Tyrosine Kinase 3 Ligand (Flt3L); Mouse FMS Like Tyrosine Kinase 3 Ligand (Flt3L) CLIA Kit; FMS Like Tyrosine Kinase 3 Ligand; FLT3LG; FLT3-LG; Flt3-L; FL; Fms-Related Tyrosine Kinase 3 ligand; SL cytokine; Flt3L clia kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of FMS Like Tyrosine Kinase 3 Ligand (Flt3L).; No significant cross-reactivity or interference between FMS Like Tyrosine Kinase 3 Ligand (Flt3L) and analogues was observed.
• Sequence Length: 232

• Applicable Applications for Flt3L clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma and other biological fluids
• Detection Range: 1.37-1,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.54pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FMS Like Tyrosine Kinase 3 Ligand (Flt3L) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FMS Like Tyrosine Kinase 3 Ligand (Flt3L) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to FMS Like Tyrosine Kinase 3 Ligand (Flt3L). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to FMS Like Tyrosine Kinase 3 Ligand (Flt3L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the FMS Like Tyrosine Kinase 3 Ligand (Flt3L) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

NCBI and Uniprot Product Information

• NCBI GI #: 227430331
• NCBI GeneID: 14256
• NCBI Accession #: NP_038548.3
• NCBI GenBank Nucleotide #: NP_038548.3
• UniProt Accession #: P49772
• NCBI Official Full Name: fms-related tyrosine kinase 3 ligand
• NCBI Official Synonym Full Names: FMS-like tyrosine kinase 3 ligand
• NCBI Official Symbol: Flt3l
• NCBI Official Synonym Symbols: Ly72L; Flt3lg
• NCBI Protein Information: fms-related tyrosine kinase 3 ligand
• UniProt Protein Name: Fms-related tyrosine kinase 3 ligand
• UniProt Gene Name: Flt3lg
• UniProt Synonym Gene Names: Flt3l; Flt3 ligand; Flt3L
• UniProt Entry Name: FLT3L_MOUSE

Uniprot Description

FLT3LG: Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Ligand, receptor tyrosine kinase; Membrane protein, integral

Cellular Component: cell surface; extracellular space

Molecular Function: protein homodimerization activity; receptor tyrosine kinase binding

Biological Process: embryonic hemopoiesis; homeostasis of number of cells within a tissue; lymphocyte differentiation; positive regulation of cell cycle; positive regulation of cell proliferation; positive regulation of myoblast differentiation; positive regulation of natural killer cell differentiation; positive regulation of protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase II promoter; regulation of myeloid dendritic cell activation