Immunohistochemistry (IHC)
(Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit anti-Human, Mouse KCNJ11 Polyclonal Antibody
KCNJ11, ID (ATP-sensitive Inward Rectifier Potassium Channel 11, IKATP, Inward Rectifier K(+) Channel Kir6.2, Potassium Channel, Inwardly Rectifying Subfamily J Member 11)
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity PurifiedPurified by immunoaffinity chromatography
Synonyms
KCNJ11; Polyclonal Antibody; ID (ATP-sensitive Inward Rectifier Potassium Channel 11; IKATP; Inward Rectifier K(+) Channel Kir6.2; Potassium Channel; Inwardly Rectifying Subfamily J Member 11); Anti -KCNJ11; anti-KCNJ11 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human KCNJ11/Kir6.2. Species Crossreactivity: Mouse
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography
Purified by immunoaffinity chromatography
Form/Format
Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Applicable Applications for anti-KCNJ11 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry.
Dilution: Immunofluorescence: 1:100-1:500
ELISA: 1:10,000
Western Blot: 1:500-1:1000
Immunohistochemistry (Paraffin): 10ug/ml
Dilution: Immunofluorescence: 1:100-1:500
ELISA: 1:10,000
Western Blot: 1:500-1:1000
Immunohistochemistry (Paraffin): 10ug/ml
Immunogen
Synthetic peptide corresponding to human KCNJ11/Kir6.2.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))
Western Blot (WB)
(Western blot analysis of extracts from 3T3 cells, using Kir6.2. The lane on the right is treated with the synthesized peptide.)
Western Blot (WB)
(Western blot analysis of extracts from 3T3 cells, using Kir6.2. The lane on the right is treated with the synthesized peptide.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells, using Kir6.2.The picture on the right is treated with the synthesized peptide.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells, using Kir6.2.The picture on the right is treated with the synthesized peptide.)
Related Product Information for anti-KCNJ11 antibody
Kir6.2/Kcnj11, an ATP sensitive inwardly-rectifying potassium channel, was originally isolated from an insulinoma library. Actual conductance requires association with the sulfonylurea receptor. This receptor is inhibited by sulfonylureas and activated by diazoxide. Mutations in the KNCJ11 gene are found in patients with permanent diabetes mellitus of infancy and hyperinsulinemic hypoglycemia. It is thought that this channel couples metabolism to membrane electrical events fibringing about insulin secretion.
Product Categories/Family for anti-KCNJ11 antibody
Similar Products
Product Notes
The KCNJ11 (Catalog #AAA645818) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KCNJ11, ID (ATP-sensitive Inward Rectifier Potassium Channel 11, IKATP, Inward Rectifier K(+) Channel Kir6.2, Potassium Channel, Inwardly Rectifying Subfamily J Member 11) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KCNJ11 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry. Dilution: Immunofluorescence: 1:100-1:500 ELISA: 1:10,000 Western Blot: 1:500-1:1000 Immunohistochemistry (Paraffin): 10ug/ml. Researchers should empirically determine the suitability of the KCNJ11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KCNJ11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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