Csk Antibody | anti-Csk antibody

Csk (Ab-364) Antibody

Cat. Num. AAA8509406

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: Csk; Csk (Ab-364) Antibody; C-SRC kinase; CSK; CYL; EC 2.7.10.2; MPK-2; kinase CSK; anti-Csk antibody

• Reactivity: Human, Mouse, Rat
• Specificity: Csk (Ab-364) antibody detects endogenous levels of total Csk protein.
• Purity/Purification: Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 450

• Applicable Applications for anti-Csk antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:20000
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human Csk around the phosphorylation site of serine 364 (K-F-SP-T-K).
• Preparation and Storage: Store at-20 degree C for 1 year.

Testing Data

(Peptide-+)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Csk (Ab-364) antibody. Western blot analysis of extracts from RAW264.7cells, treated with UV (5mins), using Csk (Ab-364) antibody.)

References

Yoshiharu Takayama, J. Biol. Chem., Jan 1999; 274: 2291. Bing Wang, Mol. Cell. Biol., Feb 2001; 21: 1077-1088. Deirdre McGarrigle, J. Biol. Chem., Apr 2006; 281: 10583-10588.

NCBI and Uniprot Product Information

• NCBI GI #: 187475373
• NCBI GeneID: 1445
• NCBI Accession #: NP_001120662.1
• NCBI GenBank Nucleotide #: NM_001127190.1
• UniProt Accession #: P41240; Q2M3N2; Q6FGZ6
• Molecular Weight: 50,704 Da
• NCBI Official Full Name: tyrosine-protein kinase CSK
• NCBI Official Synonym Full Names: C-terminal Src kinase
• NCBI Official Symbol: CSK
• NCBI Protein Information: tyrosine-protein kinase CSK
• UniProt Protein Name: Tyrosine-protein kinase CSK
• Protein Family: Chloroplast sensor kinase
• UniProt Gene Name: CSK

NCBI Description

The protein encoded by this gene is involved in multiple pathways, including the regulation of Src family kinases. It plays an important role in T-cell activation through its association with the protein encoded by the protein tyrosine phosphatase, non-receptor type 22 (PTPN22) gene. This protein also phosphorylates C-terminal tyrosine residues on multiple substrates, including the protein encoded by the SRC proto-oncogene, non-receptor tyrosine kinase gene. Phosphorylation suppresses the kinase activity of the Src family tyrosine kinases. An intronic polymorphism (rs34933034) in this gene has been found to affect B-cell activation and is associated with systemic lupus erythematosus (SLE). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2017]

Uniprot Description

Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.