Mouse Epidermal Growth Factor (EGF) CLIA Kit | EGF clia kit

Mouse Epidermal Growth Factor (EGF) CLIA Kit

Cat. Num. AAA2708802

• Gene Names: Egf; AI790464
• Reactivity: Mouse
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Epidermal Growth Factor (EGF); Mouse Epidermal Growth Factor (EGF) CLIA Kit; Epidermal Growth Factor; URG; Beta-Urogastrone; Pro-epidermal growth factor; EGF clia kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Epidermal Growth Factor (EGF).; No significant cross-reactivity or interference between Epidermal Growth Factor (EGF) and analogues was observed.
• Sequence Length: 1217

• Applicable Applications for EGF clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.69-500pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.24pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Epidermal Growth Factor (EGF) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Epidermal Growth Factor (EGF) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Epidermal Growth Factor (EGF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Epidermal Growth Factor (EGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Epidermal Growth Factor (EGF) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 170172524
• NCBI GeneID: 13645
• NCBI Accession #: NP_034243.2
• NCBI GenBank Nucleotide #: NP_034243.2
• UniProt Accession #: P01132
• NCBI Official Full Name: pro-epidermal growth factor isoform 1 preproprotein
• NCBI Official Synonym Full Names: epidermal growth factor
• NCBI Official Symbol: Egf
• NCBI Official Synonym Symbols: AI790464
• NCBI Protein Information: pro-epidermal growth factor
• UniProt Protein Name: Pro-epidermal growth factor
• Protein Family: Pro-epidermal growth factor
• UniProt Gene Name: Egf
• UniProt Synonym Gene Names: EGF
• UniProt Entry Name: EGF_MOUSE

NCBI Description

This gene encodes epidermal growth factor (EGF), the founding member of the EGF family of growth factors that are implicated in cell proliferation and differentiation. The encoded protein can localize to the membrane and function in juxtacrine signaling or undergo proteolytic processing to generate a soluble form of the hormone. Mice lacking the encoded protein do not exhibit an abnormal phenotype but transgenic mice overexpressing the encoded protein exhibit hypospermatogenesis. [provided by RefSeq, Jul 2016]

Uniprot Description

EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hormone; Ligand, receptor tyrosine kinase; Membrane protein, integral

Cellular Component: extracellular space; lysosomal membrane; plasma membrane; receptor complex

Molecular Function: epidermal growth factor receptor binding; growth factor activity; protein binding; protein-tyrosine kinase activity; Wnt receptor activity; Wnt-protein binding

Biological Process: activation of MAPK activity; activation of MAPKK activity; angiogenesis; branching morphogenesis of a tube; epidermal growth factor receptor signaling pathway; negative regulation of secretion; peptidyl-tyrosine phosphorylation; positive regulation of cell proliferation; positive regulation of DNA binding; positive regulation of epidermal growth factor receptor activity; positive regulation of fibroblast proliferation; positive regulation of granule cell precursor proliferation; positive regulation of MAP kinase activity; positive regulation of mitosis; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; positive regulation of transcription, DNA-dependent; regulation of peptidyl-tyrosine phosphorylation; regulation of protein secretion; regulation of protein transport; STAT protein nuclear translocation; Wnt receptor signaling pathway through beta-catenin