Rabbit Interleukin 6 (IL6) CLIA Kit | IL6 clia kit

Rabbit Interleukin 6 (IL6) CLIA Kit

Cat. Num. AAA2708600

• Gene Names: IL6; IL-6
• Reactivity: Rabbit
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Interleukin 6 (IL6); Rabbit Interleukin 6 (IL6) CLIA Kit; Interleukin 6; MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor; IL6 clia kit

• Reactivity: Rabbit
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 6 (IL6).; No significant cross-reactivity or interference between Interleukin 6 (IL6) and analogues was observed.
• Sequence Length: 241

• Applicable Applications for IL6 clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.69-500pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.26pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 6 (IL6) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 6 (IL6) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 6 (IL6). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 6 (IL6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 6 (IL6) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 126722667
• NCBI GeneID: 100008733
• NCBI Accession #: NP_001075533.1
• NCBI GenBank Nucleotide #: NP_001075533.1
• UniProt Accession #: Q9MZR1
• NCBI Official Full Name: interleukin-6
• NCBI Official Symbol: IL6
• NCBI Official Synonym Symbols: IL-6
• NCBI Protein Information: interleukin-6
• UniProt Protein Name: Interleukin-6
• Protein Family: Interleukin
• UniProt Gene Name: IL6
• UniProt Synonym Gene Names: IL-6

Uniprot Description

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation ().