Mouse IFT57 Monoclonal Antibody | anti-IFT57 antibody

IFT57 antibody

Cat. Num. AAA838100

• Gene Names: IFT57; HIPPI; MHS4R2; ESRRBL1
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: IFT57 antibody was purified by affinity chromatography.
• Synonyms: IFT57; Monoclonal Antibody; IFT57 antibody; Monoclonal IFT57; Anti-IFT57; Intraflagellar transport 57 homolog; HIPPI; MHS4R2; ESRRBL1; Estrogen related receptor beta like 1; Dermal papilla derived protein 8; Huntingtin interacting protein 1 interacting protein; IFT 57; IFT-57; anti-IFT57 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4F6
• Purity/Purification: IFT57 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 234

• Applicable Applications for anti-IFT57 antibody: Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:100; IHC: 1:50; WB: 1:2000
• Biological Significance: IFT57 is required for the formation of cilia. It plays an indirect role in sonic hedgehog signaling, cilia being requiredfor all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3'present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.
• Cross-Reactivity: Human
• Immunogen: IFT57 antibody was raised in mouse using a full length recombinant protein of human IFT57 (NP_060480) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant IFT57 protein (Right) or empty vector (Left) detected with IFT57 antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of IFT57 protein in paraffin embedded Adenocarcinoma of Human colon tissue using IFT57 antibody)

Related Product Information for anti-IFT57 antibody

Mouse monoclonal IFT57 antibody

Product Categories/Family for anti-IFT57 antibody

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 34783880
• NCBI GeneID: 55081
• Molecular Weight: 49,108 Da
• NCBI Official Full Name: IFT57 protein, partial
• NCBI Official Synonym Full Names: intraflagellar transport 57
• NCBI Official Symbol: IFT57
• NCBI Official Synonym Symbols: HIPPI; MHS4R2; ESRRBL1
• NCBI Protein Information: intraflagellar transport protein 57 homolog
• UniProt Protein Name: Intraflagellar transport protein 57 homolog
• Protein Family: Intraflagellar transport protein
• UniProt Gene Name: IFT57
• UniProt Synonym Gene Names: DERP8; ESRRBL1; HIPPI
• UniProt Entry Name: IFT57_HUMAN

Uniprot Description

IFT57: Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway. Has pro- apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear. Belongs to the IFT57 family.

Chromosomal Location of Human Ortholog: 3q13.13

Cellular Component: Golgi apparatus; centrosome; photoreceptor connecting cilium; cilium

Molecular Function: protein binding; DNA binding

Biological Process: regulation of apoptosis; caspase activation; smoothened signaling pathway; regulation of transcription, DNA-dependent; transcription, DNA-dependent; apoptosis; organelle organization and biogenesis; neural tube closure; intraflagellar transport; cilium biogenesis; heart looping; negative regulation of epithelial cell proliferation