Western Blot (WB)
(Anti-PQBP1 rabbit polyclonal antibody at 1:2000 dilution Lane A: OVCAR-3 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:30 kDa Observed band size:37 kDa (We are unsure as to the identity of these extra bands.))
Rabbit anti-Human PQBP1 Polyclonal Antibody | anti-PQBP1 antibody
Anti-PQBP1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PQBP1; Polyclonal Antibody; Anti-PQBP1 Antibody; Rabbit Polyclonal; PQBP1 Antibody; Rabbit PAb; Antigen Affinity Purified; polyglutamine binding protein 1; Anti-MRX2 Antibody; Anti-MRX55 Antibody; Anti-MRXS3 Antibody; Anti-MRXS8 Antibody; Anti-NPW38 Antibody; Anti-RENS1 Antibody; Anti-SHS Antibody; anti-PQBP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PQBP1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PQBP1 antibody
Western Blot (WB)
Application Notes
WB: 1:1000-1:10,000
Immunogen
E Coli-derived Human PQBP1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human PQBP1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PQBP1 rabbit polyclonal antibody at 1:2000 dilution Lane A: OVCAR-3 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:30 kDa Observed band size:37 kDa (We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-PQBP1 rabbit polyclonal antibody at 1:2000 dilution Lane A: OVCAR-3 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:30 kDa Observed band size:37 kDa (We are unsure as to the identity of these extra bands.))
Related Product Information for anti-PQBP1 antibody
Polyglutamine tract-binding protein 1 (PQBP1) is an intrinsically disordered protein composed of a small folded WW domain and a long disordered region. PQBP1 binds to spliceosomal proteins WBP11 and U5-15kD through its N-terminal WW domain and C-terminal region, respectively. PQBP1 is an RNA-splicing factor that, when mutated, in humans causes Renpenning syndrome, an X-linked intellectual disability disease characterized by severe cognitive impairment, but also by physical defects that suggest PQBP1 has broader functions in embryonic development. PQBP1 interacts with splicing-related factors via the disordered/denatured domain and is involved in transcriptional and post-transcriptional regulation of gene expression in animals, and possibly also in plants. PQBP1 in bone marrow mesenchymal stem cells may play a crucial role in bone formation and cartilage development.
References
Yang SS, et al. (2020) Pqbp1, an intellectual disability causative gene, affects bone development and growth. Biochem Biophys Res Commun 523 (4): 894-899.Wang Q, et al. (2013) Pqbp1, a factor linked to intellectual disability, affects alternative splicing associated with neurite outgrowth. Genes Dev 27 (6): 615-626.Okazawa H (2018) Pqbp1, an intrinsically disordered/denatured protein at the crossroad of intellectual disability and neurodegenerative diseases. Neurochem Int 119 17-25.Mizuguchi M, et al. (2016) Allosteric modulation of the binding affinity between pqbp1 and the spliceosomal protein u5-15kd. FEBS Lett 590 (14): 2221-2231.
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Product Notes
The PQBP1 (Catalog #AAA8108139) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PQBP1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PQBP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000-1:10,000. Researchers should empirically determine the suitability of the PQBP1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PQBP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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